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Characterization of hydroxyapatite-coated bacterial cellulose scaffold for bone tissue engineering

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Abstract

The goal of this study was to develop a novel hydroxyapatite (HA) coated bacterial cellulose (BC) scaffold for bone tissue regeneration. HA-coated BC was prepared by immersing in 30 mL of 5× simulated body fluid at 37°C for 12 h. The resulting HA-coated BC scaffolds were characterized by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATRFTIR) spectroscopy, and thermal gravimetric analysis (TGA). HA spherical globules were newly formed on the surface of the BC, and a fibrous network of BC scaffolds still maintained their dimensions for cell adhesion and proliferation. ATR-FTIR spectroscopy analysis showed bands assigned to specific signals for phosphate and carbonate ions from HA. HA-coated BC scaffolds of thermal gravimetric analysis presented residue of around 25%. The ability for bone regeneration of HA-coated BC scaffolds was evaluated using a rat calvarial defect model for 4 and 8 weeks. After implantation, both BC and HAcoated BC scaffolds showed new bone formation derived from existing bone, and found new bone even inside the scaffold. Furthermore, a new bone area was signigicantly increased in the HA-coated BC scaffolds compared with those from BC scaffolds, and bone-like materials were frequently found in HA-coated BC scaffolds. Therefore, the HA-coated BC scaffolds can be used as an effective tool for bone tissue regeneration.

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Authors and Affiliations

  1. Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Korea

    Sung-Jun Ahn, Young Min Shin, Sung In Jeong, Jin-Oh Jeong, Jong-Seok Park, Hui-Jeong Gwon, Da Eun Seo, Young-Chang Nho & Youn-Mook Lim

  2. College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Korea

    Se Eun Kim & Seong Soo Kang

  3. Department of Radiation Science and Technology, Chonbuk National University, Jeonju, 561-756, Korea

    Sung-Jun Ahn & Chong-Yeal Kim

  4. Department of Prosthodontics, School of Dentistry, Pusan National University, Dental Research Institute, Yangsan, 626-870, Korea

    Jung-Bo Huh

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  1. Sung-Jun Ahn
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Correspondence to Jung-Bo Huh or Youn-Mook Lim.

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Ahn, SJ., Shin, Y.M., Kim, S.E. et al. Characterization of hydroxyapatite-coated bacterial cellulose scaffold for bone tissue engineering. Biotechnol Bioproc E 20, 948–955 (2015). https://doi.org/10.1007/s12257-015-0176-z

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  • DOI: https://doi.org/10.1007/s12257-015-0176-z

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