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Fermentative Production of Sugarcane Vinegar by Immobilized Cells of Acetobacter aceti Under Packed Bed conditions

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Abstract

Sugarcane juice (17 °Bx), the substrate of choice for vinegar production due to its high sugar content and easy availability was fermented to ethanol by Saccharomyces cerevisiae strain 35 producing 9.5 % (v/v) ethanol. This ethanol was used for vinegar production using immobilized cells of Acetobacter aceti AC1 (on wood shavings with cell to adsorbent ratio of 2:1) packed in an indigenously developed polyvinyl chloride column. Sugarcane ethanol (supplemented with mother vinegar) with an initial acidity of 2 % (w/v) was trickled at 50 ml/h through the packed bed at 2.5 l scale, that produced sugarcane vinegar in 72 h (compared to 13 days of batch cycle). The vinegar production was consistent over 25 cycles studied and had a volatile acidity of 4.08–6.84 % (w/v), fermentation efficiency (52.9–87.9 %), acetification rate (5.8–22.8 g/l/day) and yield factor (0.68–1.14). The sugarcane vinegar had a good sensory score (8.66 ± 0.33), improved total phenols (81.9 ± 0.14–138.6 ± 0.49 mg/100 ml) with retention of ascorbic acid (42.0 ± 1.13–86.5 ± 1.34 mg/100 ml).

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References

  • Amerine, M.A., H.W. Berg, R.E. Kunkee, C.S. Ough, V.L. Singleton, and A.D. Webb. 1980. The technology of wine making, 4th ed. Westport, CT: The AVI Publishing Co.

    Google Scholar 

  • AOAC. 2000. Official methods of analysis, 17 ed., 967.21 Ascorbic acid in vitamin preparation and juice Gaithersburg, MD, USA: Association of Official Analytical Chemists.

  • Berraud, C. 2000. Production of highly concentrated vinegar in fed-batch cultures. Biotechnology Letters 22: 451–454.

    Article  CAS  Google Scholar 

  • Brodelius, P., and E.J. Vndamme. 1987. Immobilized cell systems. In Biotechnology, vol. 7(A), ed. H.J. Rehm, and G. Reed, 405–464. Weinheim: Verlag Chemie.

    Google Scholar 

  • Caputi Jr, A., and D. Wright. 1969. Collaborative study of the determination of ethanol in wine by chemical oxidation. Journal of Association of Official Analytical Chemists 52: 85–88.

    CAS  Google Scholar 

  • De Ory, I., L.E. Romero, and D. Cantero. 1999. Maximum yield acetic acid fermenter: Comparative fed-batch and continuous operation studies at pilot plant scales. Bioprocess and Biosystems Engineering 21: 187–190.

    Google Scholar 

  • De Ory, I., L.E. Romero, and D. Cantero. 2004. Optimization of immobilization conditions for vinegar production. Siran, wood chips and polyurethane foam as carriers for Acetobacter aceti. Process Biochemistry 39(5): 547–555.

    Article  Google Scholar 

  • Dombek, K.M., and L.O. Ingram. 1987. Ethanol production during batch fermentation with Saccharomyces cerevisiae: Changes in glycolytic enzymes and internal pH. Applied and Environmental Microbiology 53(6): 1286–1291.

    PubMed  CAS  Google Scholar 

  • Fregapane, G., H.R. Fernández, and M.D. Salvador. 2003. Continuous production of wine vinegar in bubble column reactors of up to 60-litre capacity. European Food Research and Technology 216: 63–67.

    CAS  Google Scholar 

  • Fu, N., and P. Peiris. 2008. Cofermentation of a mixture of glucose and xylose to ethanol by Zymomonas mobilis and Pachysolen tannophilus. World Journal of Microbiology and Biotechnology 24: 1091–1097.

    Article  CAS  Google Scholar 

  • Garg, N., D.K. Tandon, and S.K. Kalra. 1995. Production of mango vinegar by immobilized cells of Acetobacter aceti. Journal of Food Science and Technology 32(3): 216–218.

    Google Scholar 

  • Hidalgo, C., C. Vegas, E. Mateo, W. Tesfaye, A.B. Cerezo, R.M. Callejón, M. Poblet, J.M. Guillamón, A. Mas, and M.J. Torija. 2010. Effect of barrel design and the inoculation of Acetobacter pasteurianus in wine vinegar production. International Journal of Food Microbiology 141: 56–62.

    Article  PubMed  CAS  Google Scholar 

  • Joshi, N. 2010. Studies on standardization of acetic acid fermentation conditions for production of concentrated vinegar from sugarcane juice. MSc Thesis, PAU, Ludhiana.

  • Karmakar, R., A.K. Ghosh, and H. Gangopadhyay. 2011. Effect of pretreatments on physico-chemical characteristics of sugarcane juice. Sugar Tech 13(1): 47–50.

    Article  CAS  Google Scholar 

  • Kaur, M., and G.S. Kocher. 2002. Ethanol production from molasses and sugarcane juice by an adapted strain of Saccharomyces cerevisiae. Indian Journal of Microbiology 42(3): 255–257.

    Google Scholar 

  • Kaur, P., G.S. Kocher, and R.P. Phutela. 2011. Production of tea vinegar by batch and semicontinuous fermentation. Journal of Food Science and Technology 48(6): 755–758.

    Article  CAS  Google Scholar 

  • Kida, K., S. Asano, M. Yamadaki, K. Iwasaki, T. Yamaguchi, and Y. Sonoda. 1990. Continuous high ethanol fermentation from cane molasses by a flocculating yeast. Journal of Fermentation and Bioengineering 69(1): 39–45.

    Article  CAS  Google Scholar 

  • Kocher, G.S., K.L. Kalra, and R.P. Phutela. 2006. Comparative production of sugarcane vinegar by different immobilization techniques. Journal of the Institute of Brewing 112: 264–266.

    Article  CAS  Google Scholar 

  • Krusong, W., and A. Vichitraka. 2011. An air-lift acetifier with mash recycling system for corn vinegar production by Adsorbed cells of Acetobacter aceti WK on surface of loofa sponge. 2nd International conference on biotechnology and food science IPCBEE, vol. 7, 86–90.

  • Lashley, D. 1987. Alcoholic beverages making, composition and process. US Patent 4784859.

  • Lea, A.G.H. 1989. Cider vinegar. In Processed apple products, ed. D.L. Downing, 279–301. New York: Van Nostrand Reinhold.

    Chapter  Google Scholar 

  • Lotong, N., W. Malapan, A. Boongorsrang, and W. Yongmanitchai. 1989. Production of vinegar by Acetobacter cells fixed on a rotating disc reactor. Applied Microbiology and Biotechnology 32: 27–31.

    Article  CAS  Google Scholar 

  • Malik, C.P., and M. Singh. 1980. Plant enzymology and histo enzymology. Delhi: Kalyani Publishers.

    Google Scholar 

  • Marques, F.P.P., W. Spinosa, K.F. Fernandes, C.F. Castro, and M. Caliari. 2010. Quality pattern and identity of commercial fruit and vegetable vinegar (acetic acid fermentation). Ciência e Tecnologia de Alimentos 30(1): 119–126.

    Article  Google Scholar 

  • Mehaia, M.A., and M. Cheryan. 1991. Fermentation of date extracts to ethanol and vinegar in batch and continuous membrane reactors. Enzymes and Microbial Technology 13(3): 257–261.

    Article  CAS  Google Scholar 

  • Miller, C.C., Y. Park, M.W. Pariza, and M.E. Cook. 1994. Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochemical and Biophysical Research Communications 198: 1107–1112.

    Article  PubMed  CAS  Google Scholar 

  • Muhammad, A., J. Ullah, A. Muhammad, and Z. Alam. 2010. Evaluation of strawberry juice preserved with chemical preservatives at refrigeration temperature. International Journal of Nutrition and Metabolism 2(2): 27–32.

    Google Scholar 

  • Nanba, A., K. Kimura, and S. Nagai. 1985. Vinegar production by Acetobacter rancens cells fixed on a hollow fiber module. Journal of Fermentation Technology 63: 175–179.

    CAS  Google Scholar 

  • Park, Y.S., H. Ohtake, M. Fukaya, H. Okumura, Y. Kawamura, and K. Toda. 1989. Enhancement of acetic acid production in a high yield cell density culture of Acetobacter aceti. Journal of Fermentation and Bioengineering 68(5): 315–319.

    Article  CAS  Google Scholar 

  • Phanikumar, H.K. 2011. Sugarcane juice powder by spray drying technique. Science Tech Entrepreneur. www.panelamonitor.org/documents/344/sugarcane-juice-powder-spray-drying-technique. Accessed 28 Feb 2011.

  • Plata, C., C. Millan, J.C. Mauricio, and J.M. Ortega. 2003. Formation of ethyl acetate and iso-amyl acetate by various species of wine yeasts. Food Microbiology 20: 217–224.

    Article  CAS  Google Scholar 

  • Raspor, P., and D. Goranovic. 2008. Biotechnological applications of acetic acid bacteria. Critical Reviews in Biotechnology 28(2): 101–124.

    Article  PubMed  CAS  Google Scholar 

  • Sacchi, K.L., L.F. Bisson, and D.O. Adams. 2005. A review of the effect of winemaking techniques on phenolic extraction in red wines. American Journal of Enology and Viticulture 56: 197–206.

    CAS  Google Scholar 

  • Sankhla, S., A. Chaturvedi, A. Kuna, and K. Dhanlakshmi. 2012. Preservation of sugarcane juice using hurdle technology. Sugar Tech 14(1): 26–39.

    Article  CAS  Google Scholar 

  • Sossou, S.K., Y. Ameyapoh, S.D. Karou, and C.D. Souza. 2009. Study of pineapple peelings processing into vinegar by biotechnology. Pakistan Journal of Biological Sciences 11: 859–865.

    Google Scholar 

  • Tesfaye, W., M.L. Morales, M.C. García-Parrilla, and A.M. Troncoso. 2002. Wine vinegar: technology, authenticity and quality evaluation. Trends in Food Science and Technology 13: 12–21.

    Article  CAS  Google Scholar 

  • Uppal, S. 2009. Co-fermentation of xylose and glucose by Pachysolen tannophilus and Saccharomyces cerevisiae to produce ethanol. MSc Thesis, PAU, Ludhiana.

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Acknowledgments

The authors thank Department of Science and Technology, New Delhi for funding this study.

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Authors and Affiliations

  1. Department of Microbiology, Punjab Agricultural University, Ludhiana, 141004, Punjab, India

    Gurvinder Singh Kocher & Harminder Kaur Dhillon

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  1. Gurvinder Singh Kocher
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  2. Harminder Kaur Dhillon
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Correspondence to Gurvinder Singh Kocher.

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Kocher, G.S., Dhillon, H.K. Fermentative Production of Sugarcane Vinegar by Immobilized Cells of Acetobacter aceti Under Packed Bed conditions. Sugar Tech 15, 71–76 (2013). https://doi.org/10.1007/s12355-012-0179-4

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  • DOI: https://doi.org/10.1007/s12355-012-0179-4

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