Skip to main content

Advertisement

Log in

Norovirus Detection in Groundwater

  • Original Paper
  • Published:
Food and Environmental Virology Aims and scope Submit manuscript

Abstract

Waterborne disease outbreaks associated with groundwater consumption have been reported in different countries. Noroviruses are considered emerging pathogens, which cause gastroenteritis in all age groups worldwide and numerous outbreaks of noroviral gastroenteritis have been ascribed to contaminated drinking water. In Italy, few data on viral contamination of water environment and groundwater in particular are available. In this study, the presence of Norovirus GG I and GG II was investigated, using reverse transcription-polymerase chain reaction (RT-PCR) test, applied to groundwater samples collected in the Latium region in central Italy. Four out of 26 samples were positive (15.38%). Our results show both the presence of Norovirus in groundwater and the possibility to apply the RT-PCR tests for virus analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  • Anastasi, P., Bonanni, E., Cecchini, G., Divizia, M., Donia, D., Di Gianfilippo, F., et al. (2008). Rimozione dei virus nei processi convenzionali di trattamento delle acque reflue. Igiene e Sanità Pubblica, LXIV(3), 313–330.

    Google Scholar 

  • Anonymous, 2001 D. L.vo n. 31 del 2/2/2001 Gazzetta Ufficiale Serie Generale n.52 del 3-3-2001- supplemento ordinario.

  • Anonymous. (2002). UNI EN ISO 9308-1. Qualità dell’acqua—Ricerca ed enumerazione di Escherichia coli e batteri coliformi—Metodo di filtrazione su membrana. Milano: Ente Nazionale Italiano di Unificazione.

  • Anonymous. (2003). UNI EN ISO 7899-2. Qualità dell’acqua—Ricerca ed enumerazione degli enterococchi intestinali—Parte 2. Metodo di filtrazione su membrana. Milano: Ente Nazionale Italiano di Unificazione.

  • Atmar, R. L., & Estes, M. K. (2006). The epidemiologic and clinical importance of Norovirus infection. Gastroenterology Clinics of North America, 35, 275–290.

    Article  PubMed  Google Scholar 

  • Beuret, C., Kohler, D., Baumgartner, A., & Luthi, T. M. (2002). Norwalk-like virus sequences in mineral waters: One-year monitoring of three brands. Applied and Environmental Microbiology, 68, 1925–1931.

    Article  PubMed  CAS  Google Scholar 

  • Blackburn, B. G., Craun, G. F., Yoder, J. S., Hill, V., Calderon, R. L., Chen, N., et al. (2004). Surveillance for waterborne-disease outbreaks associated with drinking water—United States, 2001–2002. Morbidity and Mortality Weekly Report—Surveillance Summaries, 53, 23–45.

    PubMed  Google Scholar 

  • Blanton, L. H., Adams, S. M., Beard, R. S., Wei, G., Bulens, S. N., Widdowson, M. A., et al. (2006). Molecular and epidemiologic trends of caliciviruses associated with outbreaks of acute gastroenteritis in the United States, 2000–2004. The Journal of Infectious Disease, 193(3), 413–421.

    Article  Google Scholar 

  • Boccia, D., Tozzi, A. E., Cotter, B., Rizzo, C., Russo, T., Buttinelli, G., et al. (2002). Waterborne outbreak of Norwalk-like virus gastroenteritis at a tourist resort, Italy. Emerging Infectious Disease, 8, 563–568.

    Google Scholar 

  • Buesa, J., Collado, B., Lopèz-Andujar, P., Abu-Mallouh, R., Diaz, R. J., Diaz, A. G., et al. (2002). Molecular epidemiology of Caliciviruses causing outbreaks and sporadic cases of acute gastroenteritis in Spain. Journal of Clinical Microbiology, 40(8), 2854–2859.

    Article  PubMed  CAS  Google Scholar 

  • Carducci, A., Casini, B., Bani, A., Rovini, E., Verani, M., Mazzoni, P., et al. (2003). Virological control of groundwater quality using biomolecular tests. Water Science Technology, 47, 261–266.

    PubMed  CAS  Google Scholar 

  • Carducci, A., Morici, P., Pizzi, F., Battistin, R., Rovini, E., & Verani, M. (2008). Study of the viral removal efficiency in a urban wastewater treatment plant. Water Science Technology, 58(4), 893–897.

    Article  PubMed  CAS  Google Scholar 

  • Divizia, M., Gnesivo, C., Amore Bonapasta, R., Morace, G., Pisani, G., & Panà, A. (1993). Hepatitis A virus identification in an outbreak by enzymatic amplification. European Journal of Epidemiology, 9, 203–208.

    Article  PubMed  CAS  Google Scholar 

  • Fankhauser, R. L., Monroe, S. S., Noel, J. S., Humphrey, C. D., Bresee, J. S., Parashar, U. D., et al. (2002). Epidemiologic and molecular trends of “Norwalk-like viruses” associated with outbreaks of gastroenteritis in the United States. The Journal of Infectious Disease, 186, 1–7.

    Article  Google Scholar 

  • Formiga-Cruiz, M., Hundesa, A., Clemente-Casare, P., Albinana-Gimenez, N., Allard, A., & Girones, R. (2005). Nested multiplex PCR assay for detection of human enteric viruses in shellfish and sewage. Journal of Virological Methods, 125, 111–118.

    Article  Google Scholar 

  • Gabrieli, R., Divizia, M., Donia, D., Ruscio, V., Bonadonna, L., Diotallevi, C., et al. (1997). Evaluation of the wastewater treatment plant of Rome airport. Water Science Technology, 35(11), 193–197.

    Article  CAS  Google Scholar 

  • Gerba, C. P., Goyal, S. M., La Belle, R. L., Cech, I., & Bodgan, G. F. (1979). Failure of indicator bacteria to reflect the occurrence of enteroviruses in marine waters. American Journal of Public Health, 69, 1116–1119.

    Article  PubMed  CAS  Google Scholar 

  • Green, K. Y., Ando, T., Balayan, M. S., Berke, T., Clarke, I. N., Estes, M. K., et al. (2000). Taxonomy of the Caliciviruses. The Journal of Infectious Disease, 181(suppl 2), S322–S330.

    Article  Google Scholar 

  • Hafliger, D., Gilgen, M., Luthy, J., & Hubner, P. (1997). Seminested RT-PCR system for small round structured viruses and detection of enteric viruses in seafood. International Journal of Food Microbiology, 52, 425–429.

    Google Scholar 

  • Laverick, M. A., Wyn-Jones, A. P., & Carter, M. J. (2004). Quantitative RT-PCR for the enumeration of noroviruses (NLV) in water and sewage. Letters in Appied Microbiology, 39(2), 127–136.

    Article  CAS  Google Scholar 

  • Le Guyader, F. S., Bon, F., De Medici, D., Parnaudeau, S., Bertone, A., Crudeli, S., et al. (2006). Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption. Journal of Clinical Microbiology, 44, 3878–3882.

    Article  PubMed  Google Scholar 

  • Liang, J. L., Dziuban, E. J., Craun, G. F., Hill, V., Moore, M. R., Gelting, R. J., et al. (2006). Surveillance for waterborne disease and outbreaks associated with drinking water and water not intended for drinking—United States, 2003–2004. Morbidity and Mortality Weekly Report—Surveillance Summaries, 55, 31–65.

    PubMed  Google Scholar 

  • Locas, A., Barthe, C., Barbeau, B., Carrière, A., & Payment, P. (2007). Virus occurrence in municipal groundwater sources in Quebec, Canada. Canadian Journal Microbiology, 53, 688–694.

    Article  CAS  Google Scholar 

  • Lopman, B. A., Brown, D. W., & Koopmans, M. (2002). Human Calicivirus in Europe. Journal Clinical Virology, 24, 137–160.

    Article  CAS  Google Scholar 

  • Lopman, B. A., Reacher, M. H., van Duijnhoven, Y., Hanon, F. X., Brown, D., & Koopmans, M. (2003). Viral gastroenteritis outbreaks in Europe, 1995–2000. Emerging Infectious Disease, 9, 90–96.

    Google Scholar 

  • Lucena, F., Ribas, F., Duran, A. E., Skraber, S., Gantzer, C., Campos, C., et al. (2006). Occurence of bacterial indicators and bacteriophages infecting enteric bacteria in groundwater in different geographical areas. Journal Applied Microbiology, 101, 96–102.

    Article  CAS  Google Scholar 

  • Lynch, M., Painter, J., Woodruff, R., & Braden, C. (2006). Surveillance for foodborne-disease outbreaks—United States, 1998–2002. Morbidity and Mortality Weekly Report—Surveillance Summaries, 55, 1–42.

    PubMed  Google Scholar 

  • Martinelli, D., Prato, R., Chironna, M., Sallustio, A., Caputi, G., Conversano, M., et al. (2007). Large outbreak of viral gastroenteritis caused by contaminated drinking water in Apulia, Italy, May–October 2006. Eurosurveillance, 12(4), E070419.1.

    PubMed  CAS  Google Scholar 

  • Maunula, L., Miettinen, I. T., & von Bonsdorff, C. H. (2005). Norovirus outbreaks from drinking water. Emerging Infectious Disease, 11, 1716–1721.

    Google Scholar 

  • Maurer, A. M., & Sturchler, D. (2000). A waterborne outbreak of small round structured virus, campylobacter and shigella co-infections in La Neuveville, Switzerland, 1998. Epidemiology and Infection, 125, 325–332.

    Article  PubMed  CAS  Google Scholar 

  • Montagna, M. T., Signorile, G., De Donno, A., Bagordo, F., & Carrozzini, F. (1997). Groundwater quality in Southern Salento. Journal of Preventive Medicine and Hygiene, 38, 5–9.

    Google Scholar 

  • Personné, J. C., Poty, F., Vaute, L., & Drogue, C. (1998). Survival, transport and dissemination of Escherichia coli and enterococci in a fessurated environment. Study of a flood in a karstic aquifer. Journal of Applied Microbiology, 84, 431–438.

    Article  PubMed  Google Scholar 

  • Poullis, D. A., Attwell, R. W., & Powell, S. C. (2002). An evaluation of waterborne-disease surveillance in the European Union. Reviews on Environmental Health, 17, 149–161.

    PubMed  Google Scholar 

  • Prato, R., Lopalco, P. L., Chironna, M., Barbuti, G., Germinario, C., & Quarto, M. (2004). Norovirus gastroenteritis general outbreak associated with raw shellfish consumption in South Italy. BMC Infectious Disease, 4, 37.

    Article  Google Scholar 

  • Rizzo, C., Di Bartolo, I., Santantonio, M., Coscia, M. F., Monno, R., De Vito, D., et al. (2007). Epidemiological and virological investigation of a Norovirus outbreak in a resort in Puglia, Italy. BMC Infectious Diseases, 7, 135.

    Article  PubMed  Google Scholar 

  • Yoder, J., Roberts, V., Craun, G. F., et al. (2008). Surveillance for waterborne disease and outbreaks associated with drinking water and water not intended for drinking—United States, 2005–2006. Morbidity and Mortality Weekly Report—Surveillance Summaries, 57(No SS-9), 39–69.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. Gabrieli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gabrieli, R., Maccari, F., Ruta, A. et al. Norovirus Detection in Groundwater. Food Environ Virol 1, 92–96 (2009). https://doi.org/10.1007/s12560-009-9014-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12560-009-9014-9

Keywords

Navigation