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The Impact of Combined Sewage Overflows on the Viral Contamination of Receiving Waters

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Abstract

The contribution of combined sewer overflows (CSO) to the viral contamination of receiving waters was determined. Adenovirus concentrations were determined using the Primary Liver Carcinoma (PLC/PRF/5) cell line and confirmed by Polymerase Chain Reaction (PCR). Norovirus concentration was determined using the Most Probable Number (MPN) and Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Seventy-five water samples were collected during dry weather and 50 samples were collected during wet weather. CSO events significantly increased the concentration of culturable viruses, adenoviruses, and noroviruses in the receiving waters (P < 0.01). During dry weather, 56% of samples were positive for total virus cytopathic effects (CPE), adenoviruses were detected in 41% of the positive cell cultures, and noroviruses in 6% of the concentrates by direct RT-PCR. During wet weather, 100% of the samples were positive by CPE, 84% for adenoviruses, and 40% in the concentrates for norovirus. Our results demonstrate that CSOs can contribute significant viral loading to receiving waters.

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Acknowledgments

Special thanks to Francisco Calderon and Sara Beck for their comments on this manuscript.

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Author notes

  1. Roberto A. Rodríguez

    Present address: Department of Civil, Environmental and Architectural Engineering, University of Colorado at Boulder, Boulder, CO, USA

Authors and Affiliations

  1. Department of Soil, Water and Environmental Science, University of Arizona, P.O. Box 210038, Tucson, AZ, 8572, USA

    Roberto A. Rodríguez, Patricia M. Gundy & Charles P. Gerba

  2. Metropolitan Water Reclamation District of Greater Chicago, Cicero, IL, USA

    Geeta K. Rijal

Authors
  1. Roberto A. Rodríguez
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  2. Patricia M. Gundy
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  3. Geeta K. Rijal
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  4. Charles P. Gerba
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Corresponding author

Correspondence to Charles P. Gerba.

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Rodríguez, R.A., Gundy, P.M., Rijal, G.K. et al. The Impact of Combined Sewage Overflows on the Viral Contamination of Receiving Waters. Food Environ Virol 4, 34–40 (2012). https://doi.org/10.1007/s12560-011-9076-3

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  • DOI: https://doi.org/10.1007/s12560-011-9076-3

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