Skip to main content
SpringerLink
Log in

Tyrosinase Inhibitory and Anti-oxidative Effects of Lactic Acid Bacteria Isolated from Dairy Cow Feces

  • Published:
Probiotics and Antimicrobial Proteins Aims and scope Submit manuscript

Abstract

Overproduction and accumulation of melanin cause a number of skin diseases. The inhibitors of tyrosinase are important for the treatment of skin diseases associated with hyper-pigmentation after UV exposure and application in cosmetics for whitening and depigmentation. Reactive oxygen species (ROS) including hydrogen peroxide are generated by chemical substances and metabolic intermediates and cause various diseases including cancer and heart diseases. We have isolated four different lactic acid bacteria (LAB) strains from dairy cow feces and investigated the tyrosinase inhibition and anti-oxidative effects of culture filtrates prepared from the isolated bacteria, which are designated as EA3, EB2, PC2, and PD3. To investigate optimal culture conditions isolated LAB strains, the measurements of tyrosinase inhibitory and anti-oxidative activities were performed. The results of tyrosinase inhibitory activities revealed that Enterococcus sp. EA3 showed about 65% at culture conditions (14 h, 30 °C, pH 8, and 0% NaCl), Enterococcus sp. EB2 about 65% at culture conditions (12 h, 30 °C, pH 9, and 0% NaCl), Pediococcus sp. PC2 about 80% at culture conditions (20 h, 30 °C, pH 6, and 0% NaCl), and Pediococcus sp. PD3 about 80% at culture conditions (20 h, 30 °C, pH 8, and 0% NaCl), respectively. In addition, anti-oxidative activities against four different LAB strains showed approximately more than 30% at optimal conditions for the measurements of tyrosinase inhibitory activities. From the results, we have suggested that the isolated four LAB strains could be useful for a potential agent for developing anti-oxidants and tyrosinase inhibitors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Chang T-S (2009) An updated review of tyrosinase inhibitors. Int J Mol Sci 10(6):2440–2475

    Article  CAS  Google Scholar 

  2. Parvez S, Kang M, Chung HS, Bae H (2007) Naturally occurring tyrosinase inhibitors: mechanism and applications in skin health, cosmetics and agriculture industries. Phytother Res 21(9):805–816

    Article  CAS  Google Scholar 

  3. Sánchez-Ferrer Á, Rodríguez-López JN, García-Cánovas F, García-Carmona F (1995) Tyrosinase: a comprehensive review of its mechanism. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology 1247(1):1–11

    Article  Google Scholar 

  4. Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S (2003) Glabrene and isoliquiritigenin as tyrosinase inhibitors from licorice roots. J Agric Food Chem 51(5):1201–1207

    Article  CAS  Google Scholar 

  5. Fu B, Li H, Wang X, Lee FS, Cui S (2005) Isolation and identification of flavonoids in licorice and a study of their inhibitory effects on tyrosinase. J Agric Food Chem 53(19):7408–7414

    Article  CAS  Google Scholar 

  6. Gadd G (1980) Melanin production and differentiation in batch cultures of the polymorphic fungus Aureobasidium pullulans. FEMS Microbiol Lett 9(3):237–240

    Article  CAS  Google Scholar 

  7. Bell AA, Wheeler MH (1986) Biosynthesis and functions of fungal melanins. Annu Rev Phytopathol 24(1):411–451

    Article  CAS  Google Scholar 

  8. Zimmerman W, Blanchette R, Burnes T, Farrell R (1995) Melanin and perithecial development in Ophiostoma piliferum. Mycologia:857–863

  9. Priestley GC (1993) Molecular aspects of dermatology, vol 3. Wiley

  10. Martinez MV, Whitaker JR (1995) The biochemistry and control of enzymatic browning. Trends Food Sci Technol 6(6):195–200

    Article  CAS  Google Scholar 

  11. Funasaka Y, Komoto M, Ichihashi M (2000) Depigmenting effect of α-tocopheryl ferulate on normal human melanocytes. Pigment Cell Res 13(s8):170–174

    Article  Google Scholar 

  12. Rackova L, Oblozinsky M, Kostalova D, Kettmann V, Bezakova L (2007) Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. J Inflamm 4(1):1

    Article  Google Scholar 

  13. Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408(6809):239–247

    Article  CAS  Google Scholar 

  14. Senthil K, Aranganathan S, Nalini N (2004) Evidence of oxidative stress in the circulation of ovarian cancer patients. Clin Chim Acta 339(1):27–32

    Article  CAS  Google Scholar 

  15. Galvan I, Solano F (2009) The evolution of eu-and pheomelanic traits may respond to an economy of pigments related to environmental oxidative stress. Pigment cell & melanoma research 22(3):339–342

    Article  CAS  Google Scholar 

  16. Galván I, Alonso-Alvarez C (2009) The expression of melanin-based plumage is separately modulated by exogenous oxidative stress and a melanocortin. Proc R Soc Lond B Biol Sci 276(1670):3089–3097

    Article  Google Scholar 

  17. Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, Hearing VJ, Lozano JA, Garcia-Borron JC (1994) A new enzymatic function in the melanogenic pathway. The 5, 6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1). J Biol Chem 269(27):17993–18000

    CAS  Google Scholar 

  18. Giraffa G, Chanishvili N, Widyastuti Y (2010) Importance of lactobacilli in food and feed biotechnology. Res Microbiol 161(6):480–487

    Article  Google Scholar 

  19. Arihara K, Ota H, Itoh M, Kondo Y, Sameshima T, Yamanaka H, Akimoto M, Kanai S, Miki T (1998) Lactobacillus acidophilus group lactic acid bacteria applied to meat fermentation. J Food Sci 63(3):544–547

    Article  CAS  Google Scholar 

  20. Hammes W, Hertel C (1998) New developments in meat starter cultures. Meat Sci 49:S125–S138

    Article  Google Scholar 

  21. Pomerantz SH (1963) Separation, purification, and properties of two tyrosinases from hamster melanoma. J Biol Chem 238(7):2351–2357

    CAS  Google Scholar 

  22. Dietz BM, Kang Y-H, Liu G, Eggler AL, Yao P, Chadwick LR, Pauli GF, Farnsworth NR, Mesecar AD, van Breemen RB (2005) Xanthohumol isolated from Humulus lupulus inhibits menadione-induced DNA damage through induction of quinone reductase. Chem Res Toxicol 18(8):1296–1305

    Article  CAS  Google Scholar 

  23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26(9):1231–1237

    Article  CAS  Google Scholar 

  24. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47(3):469–474

    Article  CAS  Google Scholar 

  25. Lim TY, Lim YY, Yule CM (2009) Evaluation of antioxidant, antibacterial and anti-tyrosinase activities of four Macaranga species. Food Chem 114(2):594–599

    Article  CAS  Google Scholar 

  26. Fawole OA, Makunga NP, Opara UL (2012) Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract. BMC Complement Altern Med 12(1):1

    Article  Google Scholar 

  27. Lai HY, Lim YY, Tan SP (2009) Anti-oxidative, tyrosinase inhibiting and antibacterial activities of leaf extracts from medicinal ferns. Biosci Biotechnol Biochem 73(6):1362–1366

    Article  CAS  Google Scholar 

  28. Huang H-C, Huang W-Y, Chiu S-H, Ke H-J, Chiu S-W, Wu S-Y, Kuo F-S, Chang T-M (2011) Antimelanogenic and anti-oxidative properties of Bifidobacterium bifidum. Arch Dermatol Res 303(7):527–531

    Article  Google Scholar 

  29. Li S, Zhao Y, Zhang L, Zhang X, Huang L, Li D, Niu C, Yang Z, Wang Q (2012) Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Food Chem 135(3):1914–1919

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Research Grant of Pukyong National University (year 2016).

Author information

Authors and Affiliations

  1. Department of Microbiology, Pukyong National University, Busan, 48513, South Korea

    Keunho Ji, Youn Su Cho & Young Tae Kim

Authors
  1. Keunho Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Youn Su Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Young Tae Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Young Tae Kim.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ji, K., Cho, Y.S. & Kim, Y.T. Tyrosinase Inhibitory and Anti-oxidative Effects of Lactic Acid Bacteria Isolated from Dairy Cow Feces. Probiotics & Antimicro. Prot. 10, 43–55 (2018). https://doi.org/10.1007/s12602-017-9274-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12602-017-9274-x

Keywords

Search

Navigation