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Twelve tetranucleotide microsatellite loci for westslope cutthroat trout Oncorhynchus clarki lewisi (Salmonidae)

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Abstract

We isolated 12 tetra-nucleotide microsatellite loci from westslope cutthroat trout (Oncorhynchus clarki lewisi). These loci were tested against 58 individuals from a single creek for polymorphism. The number of alleles ranged from 2 to 8, with an average of 4.3. The expected heterozygosity ranged from 0.12 to 0.79, with an average of 0.52. Ten of the twelve loci conformed to Hardy–Weinberg expectations. These microsatellite loci will be useful for describing population structure in westslope cutthroat trout.

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Acknowledgements

This work was funded by Montana Fish Wildlife and Parks, and the National Science Foundation (DEB 0717456). We would like to thank Ingrid Spies (National Oceanic Atmospheric Administration, Seattle, Washington) for providing us with the facility to perform our enrichment.

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  1. Department of Ecology, Montana State University, 310 Lewis Hall, Bozeman, MT, 59717, USA

    Ninh V. Vu & Steven T. Kalinowski

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  1. Ninh V. Vu
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  2. Steven T. Kalinowski
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Correspondence to Steven T. Kalinowski.

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Vu, N.V., Kalinowski, S.T. Twelve tetranucleotide microsatellite loci for westslope cutthroat trout Oncorhynchus clarki lewisi (Salmonidae). Conservation Genet Resour 1, 249–251 (2009). https://doi.org/10.1007/s12686-009-9061-4

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  • DOI: https://doi.org/10.1007/s12686-009-9061-4

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