Abstract
To explore a new and simple rapid extraction and purification technique for wheat-esterase, an ionic liquids (ILs)-based aqueous two-phase system (ATPS) was developed for the purification of wheat-esterase from wheat extracts. Effects of various process parameters such as the concentrations of [Bmim]BF4, the types and concentrations of phase-forming salt, the system pH and the temperature on partitioning of wheat-esterase were evaluated. The obtained data indicated that wheat-esterase was preferentially partitioned into the ILs-rich phase and the ATPS composed of 20 % [Bmim]BF4 (w/w) and 25 % (w/w) NaH2PO4(pH = 4.8) showed good selectivity on wheat-esterase. Under the optimum conditions, wheat-esterase was purified with an acceptable yield (88.93 %), but produced wheat-esterase was 4.23 times as pure. It was obvious that temperature shows little influence on the purification between 10 and 50 °C. Sephadex G-150FF revealed that the band intensity of contaminating proteins in ATPS fraction almost disappeared. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of wheat-esterase from wheat crude extracts.
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Acknowledgments
The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (No.31201366), Science and technology research projects of Education Department of Heilongjiang Province (No.11551063).
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Jiang, B., Feng, Z., Liu, C. et al. Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt. J Food Sci Technol 52, 2878–2885 (2015). https://doi.org/10.1007/s13197-014-1319-5
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DOI: https://doi.org/10.1007/s13197-014-1319-5