Abstract
Large amount of cassava pulp is produced as by-product of industrial tapioca production. The value-added process of this low-cost waste is to use it as a substrate for bioethanol production. However, during the pulp pretreatment by acidification combined with steam explosion, many yeast inhibitors including acetic acid, formic acid, levulinic acid, furfural and 5-hydroxymethylfurfural are generated and these compounds have negative effects on the subsequent fermentation step. Therefore, the objective of this study was to investigate whether the repeated cultures of Saccharomyces cerevisiae SC90 could alleviate this problem. To obtain the inhibitor tolerable cells, the repeated culture was performed by growing yeast cells to a specific growth rate (µ) of 0.22 h−1 or higher (80% of the µ in control) and then transferring them to progressively higher concentrations of hydrolysate ranging from 20 to 100% (v/v). The results showed a tendency of longer lag phase as well as time to reach maximum cell number (tmaxc) with an increase in hydrolysate concentration. However, the repeated culture at the same hydrolysate concentration could shorten both lag period and tmaxc. Interestingly, the growth and fermentation efficiency of adapted cells in 100% hydrolysate were significantly higher (p ≤ 0.05) than those of non-adapted cells by 38% and 27%, respectively.
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Acknowledgements
This research was financially supported by King Mongkut’s Institute of Technology Ladkrabang (KMITL) (A118-59-070) and National Research Council of Thailand. The contribution of Prof. Dr. Anthony Keith Thompson and Prof. Dr. George Srzednicki (visiting Professor at Faculty of Agro-Industry, KMITL) for critically editing the manuscript is acknowledged.
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PPNA: literature review, design of the research outline, design of research tools, analysis of data, interpretation of the data analysis, and drafting of the article (first draft). TC: design of the research outline, design of research tools, decision on the data analysis method, interpretation of the data analysis, revision of the draft of the article (second draft), and final approval of the article (final draft). WK: conception of the project, design of the research outline, interpretation of the data analysis, and final approval of the article (final draft). SP: conception of the project, literature review, design of the research outline, design of research tools, decision on the data analysis method, analysis of data, interpretation of the data analysis, revision of the draft of the article (second draft), and final approval of the article (final draft).
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Palakawong Na Ayutthaya, P., Charoenrat, T., Krusong, W. et al. Repeated cultures of Saccharomyces cerevisiae SC90 to tolerate inhibitors generated during cassava processing waste hydrolysis for bioethanol production. 3 Biotech 9, 76 (2019). https://doi.org/10.1007/s13205-019-1607-x
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DOI: https://doi.org/10.1007/s13205-019-1607-x