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Fermentation of sago starch to biobutanol in a batch culture using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

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Abstract

Acetone–butanol–ethanol (ABE) was produced in Clostridium saccharoperbutylacetonicum N1-4 using sago starch as a substrate, and the effects of nutrient supplementation on ABE production were studied. Using sago starch as a substrate led to higher butanol production in C. saccharoperbutylacetonicum N1-4 when compared to other starchy materials as substrates. TYA medium supplemented with sago starch led to enhanced ABE and butanol production; however, P2 medium improved ABE production more than TYA medium. The highest ABE and butanol production was achieved with P2 medium supplemented with 70 g/L of sago starch. Using these fermentation conditions, ABE and butanol were produced at 16.65 and 9.83 g/L, respectively, and productivity was 0.15 g/L h for ABE and 0.09 g/L h for butanol. Using P2 medium supplemented with either 50 g/L enzymatically hydrolyzed sago starch or 70 g/L sago starch without hydrolysis, 9.81 and 9.83 g/L butanol were produced, respectively. These results reveal the importance of media formulation in the biobutanol fermentation of sago starch by C. saccharoperbutylacetonicum N1-4.

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Acknowledgments

We would like to thank Prof. Dr Yoshino Sadazo, Kyushu University, Japan, who provided us with C. saccharoperbutylacetonicum N1-4. This research was supported by the UKM-GUP-KPB-08-32-128 grant.

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Authors and Affiliations

  1. School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia

    Najeeb Kaid Nasser Al-Shorgani & Wan Mohtar Wan Yusoff

  2. Department of Chemical and Process Engineering, Faculty of Engineering, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia

    Mohd Sahaid Kalil

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  1. Najeeb Kaid Nasser Al-Shorgani
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  2. Mohd Sahaid Kalil
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  3. Wan Mohtar Wan Yusoff
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Correspondence to Najeeb Kaid Nasser Al-Shorgani.

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Al-Shorgani, N.K.N., Kalil, M.S. & Yusoff, W.M.W. Fermentation of sago starch to biobutanol in a batch culture using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). Ann Microbiol 62, 1059–1070 (2012). https://doi.org/10.1007/s13213-011-0347-x

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