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Decolorization of chemically different dyes by white-rot fungi in submerged cultures

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Abstract

Forty-two white-rot fungi in submerged cultures were tested to determine their dye decolorization capacity and the optimal conditions for the decolorization process. Trametes pubescens Cui 7571 was found to be the most effective strain in terms of decolorization performance on the azo dye Congo Red, and it exhibited excellent reusability as well as persistence in sequential decolorization experiments. Optimization of the decoloration process was also conducted to evaluate the effects of a number of chemical compounds, metal salts, inducers, and mediators on the dye decolorization rate. On the seventh day, a highest dye removal of 98.83 % was observed with addition of copper at 2.5 mmol L−1, Tween 80 at 1.0 % (v/v), and ferulic acid at 0.50 μmol L−1, respectively. The adsorption of mycelia to dyes was not a significant contributor to dye removal, and decolorization by the functional fungus T. pubescens depended on biodegradation by enzymes, as evidenced by the results of the moist heat sterilization treatment (121°C for 20 min), induction of extracellular enzymes, and scanning electron microscopy. Four dye degradation metabolites, i.e., naphthalene amine, biphenyl amine, biphenyl ,and naphthalene diazonium, were identified by Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The phytotoxicity tests indicated that degraded metabolites had almost a negligible effect on the plant seeds as compared to that of dye, which is indicative of the less toxic nature of the metabolites. Our results suggest that white-rot fungus T. pubescens could be developed into a novel azo dye bioremediation strategy.

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Acknowledgments

The study was supported by the Program for New Century Excellent Talents in University (NCET-11-0585).

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Authors and Affiliations

  1. Institute of Microbiology, Beijing Forestry University, Qinghuadong Road 35, P.O. Box 61, Haidian District, Beijing, 100083, People’s Republic of China

    Jing Si, Bao-Kai Cui & Yu-Cheng Dai

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  1. Jing Si
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  2. Bao-Kai Cui
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  3. Yu-Cheng Dai
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Correspondence to Yu-Cheng Dai.

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Si, J., Cui, BK. & Dai, YC. Decolorization of chemically different dyes by white-rot fungi in submerged cultures. Ann Microbiol 63, 1099–1108 (2013). https://doi.org/10.1007/s13213-012-0567-8

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