Skip to main content
SpringerLink
Log in

Isolation and characterization of trichalasin-producing endophytic fungus from Taxus baccata

  • Original Article
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

An endophytic fungus (strain T1) isolated from Taxus baccata was studied for the production of metabolites with anticancer and antioxidant activities. This fungus was identified as Diaporthe sp. based on rDNA-internal transcribed spacer (ITS) sequence analysis. The crude extract showed cytotoxic activity against MCF-7 and HeLa cancer cell lines, with IC50 (concentration inhibiting 50% of growth rate) values of 1058 ± 44 and 1257 ± 80 μg ml−1, respectively. The scavenging activity of fungal extract increased significantly with increasing concentration [IC50 (concentration required to scavenge 50% of free radicals) 482 ± 9 μg ml−1]. Ultra-high-performance liquid chromatography-quadrupole-time of flight analysis revealed the presence of three trichalasins (trichalasin E, F and H) in the crude extract of T1 which are known to have antitumour and antioxidant activities. These results suggest that Diaporthe sp. has the potential to be used for therapeutic purposes because of its antiproliferative and antioxidant potential and also for the production of cytochalasins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Agusta A, Ohashi K, Shibuya H (2006) Bisanthraquinone metabolites produced by the endophytic fungus Diaporthe sp. Chem Pharm Bull 54:579–582

    Article  CAS  PubMed  Google Scholar 

  • Ascêncio PGM, Ascêncio SD, Aguiar AA, Fiorini A, Pimenta RS (2014) Chemical assessment and antimicrobial and antioxidant activities of endophytic fungi extracts isolated from Costus spiralis (Jacq.) Roscoe (Costaceae). Evidence-based complementary and alternative medicine. Article ID 190543. doi:10.1155/2014/190543

  • Bacon CW, White JF (2000) Microbial endophytes. Marcel Deker Inc., New York: CRC Press

  • Cabezas L, Calderon C, Medina LM, Bahamon I, Cardenas M, Bernal AJ, Gonzalez A, Restrepo S (2012) Characterization of cellulases of fungal endophytes isolated from Espeletia spp. J Microbiol 50:1009–1013

    Article  CAS  PubMed  Google Scholar 

  • Carvalho CR, Gonçalves VN, Pereira CB, Johann S, Galliza IV, Alves TMA, Rabello A, Sobral MEG, Zani C, Rosa CA, Rosa LH (2012) The diversity, antimicrobial and anticancer activity of endophytic fungi associated with the medicinal plant Stryphnodendron adstringens (Mart.) Coville (Fabaceae) from the Brazilian savannah. Symbiosis 57:95–107

    Article  Google Scholar 

  • Casella TM, Eparvier V, Mandavid H, Bendelac A, Odonne G, Dayan L, Duplais C, Espindola LS, Stien D (2013) Antimicrobial and cytotoxic secondary metabolites from tropical leaf endophytes: Isolation of antibacterial agent pyrrocidine C from Lewia infectoria SNB-GTC2402. Phytochemistry 96:370–377

    Article  CAS  PubMed  Google Scholar 

  • Chen L, Liu YT, Song B, Zhang HW, Ging G, Liu XZ, Gu YC, Zou ZM (2014) Stereochemical determination of new cytochalasins from the plant endophytic fungus Trichoderma gamsii. Fitoterapia 96:115–122

    Article  CAS  PubMed  Google Scholar 

  • Corrêa RC, Rhoden SA, Mota TR, Azevedo JL, Pamphile JA, de Souza CG, Polizeli Mde L, Bracht A, Peralta RM (2014) Endophytic fungi: expanding the arsenal of industrial enzyme producers. J Ind Microbiol Biotechnol 41:1467–1478

    Article  PubMed  Google Scholar 

  • Denizot F, Lang R (1986) Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Method 89:271–277

    Article  CAS  Google Scholar 

  • Deshmukh SK, Verekar SA, Bhave SV (2015) Endophytic fungi: a reservoir of antibacterials. Front Microbiol 8:715. doi:10.3389/fmicb.2014.00715

  • Dos Santos TT, de Souza LT, de Queiroz CB, de Araújo EF, Pereira OL, de Queiroz MV (2016) High genetic variability in endophytic fungi from the genus Diaporthe isolated from common bean (Phaseolus vulgaris L.) in Brazil. J Appl Microbiol 120:388–401

    Article  PubMed  Google Scholar 

  • Eaton CJ, Cox MP, Scott B (2011) What triggers grass endophytes to switch from mutualism to pathogenism? Plant Sci 180:190–195

    Article  CAS  PubMed  Google Scholar 

  • Eriksson OE, Vue JZ (1998) Bambusicolous pyrenomycetes, an annotated check-list. Myconet 1:25–78

  • Garyali S, Kumar A, Reddy MS (2013) Taxol production by an endophytic fungus, Fusarium redolens, isolated from Himalayan yew. J Microbiol Biotechnol 23:1372–1380

    Article  CAS  PubMed  Google Scholar 

  • Guo LD, Hyde KD, Liew ECY (2000) Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol 147:617–630

    Article  CAS  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kharwar RN, Mishra A, Gond SK, Stierle A, Stierle D (2011) Anticancer compounds derived from fungal endophytes: their importance and future challenges. Nat Prod Rep 28:1208–228

    Article  CAS  PubMed  Google Scholar 

  • Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN (2002) Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 13:8–17

    Article  CAS  PubMed  Google Scholar 

  • Lin X, Huang Y, Fang M, Wang J, Zheng Z, Su W (2005) Cytotoxic and antimicrobial metabolites from marine lignicolous fungi, Diaporthe sp. FEMS Microbiol Lett 251:53–58

    Article  CAS  PubMed  Google Scholar 

  • Liu K, Ding X, Deng B, Chen W (2009) Isolation and characterization of endophytic taxol-producting fungi Taxus chinensis. J Ind Microbiol 36:1171–1177

    Article  CAS  Google Scholar 

  • Naik BS, Shashikala J, Krishnamurthy YL (2008) Diversity of fungal endophytes in shrubby medicinal plants of Malnad region, Western Ghats, Southern India. Fungal Ecol 1:89–93

    Article  Google Scholar 

  • Nath A, Raghunatha P, Joshi SR (2012) Diversity and biological activities of endophytic fungi of Emblica officinalis, an ethnomedicinal plant of India. Mycobiology 40:8–13

    Article  PubMed  PubMed Central  Google Scholar 

  • Nisa H, Kamili AN, Nawchoo IA, Shafi S, Shameem N, Bandh SA (2015) Fungaal endophytesas prolific source of phytochemicals and other bioactive natural products: A review. Microb Pathog 82:50–59

    Article  CAS  PubMed  Google Scholar 

  • Pornpakakul S, Roengsumran S, Deechangvipart S, Petsom A, Muangsin N, Ngamrojnavanich N, Sriubolmas N, Chaichit N, Ohta T (2007) Diaporthichalasin, a novel CYP3A4 inhibitor from an endophytic Diaporthe sp. Tetrahedron Lett 48:651–655

    Article  CAS  Google Scholar 

  • Priti V, Ramesha BT, Singh S, Ravikanth G, Ganeshaiah KN, Suryanarayanan TS, Shaanker RU (2009) How promising are endophytic fungi as alternative sources of plant secondary metabolites? Curr Sci 97:477–478

    Google Scholar 

  • Rukachaisirikul V, Sommart U, Phongpaichit S, Sakayaroj J (2008) Metabolites from the endophytic fungus Phomopsis sp. PSU-D15. Phytochemistry 69:783–787

    Article  CAS  PubMed  Google Scholar 

  • Scherlach K, Boettger D, Remme N, Hertweck C (2010) The chemistry and biology of cytochalasans. Nat Prod Rep 27:869–886

    Article  CAS  PubMed  Google Scholar 

  • Schulz B, Boyle C (2005) The endophytic continuum. Mycol Res 109:661–686

    Article  PubMed  Google Scholar 

  • Silva GH, Teles HL, Trevisan HC, Young MCM, Pfenning LH, Eberlin MN, Haddad R, Costa-Neto CM, Bolzani VS, Araújo AR (2005) New bioactive metabolites produced by Phomopsis cassiae, an endophytic fungus in Cassia spectabilis. J Braz Chem Soc 16:1463–1466

    Article  CAS  Google Scholar 

  • Soca-Chafre G, Rivera-Orduña FN, Hidalgo-Lara ME, Hernandez-Rodriguez C, Marsch R, Flores-Cotera LB (2011) Molecular phylogeny and paclitaxel screening of fungal endophytes from Taxus globosa. Fungal Biol 111:143–156

    Article  Google Scholar 

  • Strobel G, Daisy B (2003) Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Res 67:491–502

    Article  CAS  Google Scholar 

  • Suryanarayanan TS, Thirunavukkarasu N, Govindarajulu MB, Sasse F, Jensen R, Murali TS (2009) Fungal endophytes and bioprospecting. Fungal Biol Rev 23:9–19

    Article  Google Scholar 

  • Tejesvi MV, Nalini MS, Mahesh B, Parkash SH, Kinni RK, Shetty HS, Subbiah V (2007) New hopes from endophytic fungal secondary metabolite. Bol Soc Quim Mex 1:19–26

    Google Scholar 

  • Van Niekerk JM, Groenewald JZ, Farr DF, Fourie PH, Halleer F, Crous PW (2005) Reassessment of Phomopsis species on grapevines. Australas Plant Pathol 34:1–13

    Article  Google Scholar 

  • Vieira ML, Hughes AF, Gil VB, Vaz AB, Alves TM, Zani CL, Rosa CA, Rosa LH (2012) Diversity and antimicrobial activities of the fungal endophyte community associated with the traditional Brazilian medicinal plant Solanum cernuum Vell. (Solanaceae). Can J Microbiol 58:1–13

    Article  Google Scholar 

  • Wagenaar MM, Corwin J, Strobel G, Clardy J (2000) Three new cytochalasins produced by an endophytic fungus in the Genus Rhinocladiella. J Nat Prod 63:1692–1695

    Article  CAS  PubMed  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and application. Academic, San Diego, pp 315–322

    Google Scholar 

  • Yan BC, Wang WG, Hu DB, Sun X, Kong LM, Li XN, Du X, Luo SH, Liu Y, Li Y, Sun HD, Pu JX (2016) Phomopchalasins A and B, two cytochalasans with polycyclic-fused skeletons from the endophytic fungus Phomopsis sp. shj2. Org Lett 18:1108–1111

    Article  CAS  PubMed  Google Scholar 

  • Zhang P, Zhou PP, Jiang C, Yu H, Yu LJ (2008) Screening of taxol-producing fungi based on PCR amplification from Taxus. Biotechnol Lett 30:2119–2123

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Thapar University, Patiala, 147004, Punjab, India

    Mondem Vasundhara, Manoj Baranwal & Anil Kumar

  2. Nano Temper Technologies, World Trade Centre, Bangalore, 560055, India

    Nallapeta Sivaramaiah

Authors
  1. Mondem Vasundhara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manoj Baranwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nallapeta Sivaramaiah
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anil Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mondem Vasundhara.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vasundhara, M., Baranwal, M., Sivaramaiah, N. et al. Isolation and characterization of trichalasin-producing endophytic fungus from Taxus baccata . Ann Microbiol 67, 255–261 (2017). https://doi.org/10.1007/s13213-017-1256-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13213-017-1256-4

Keywords

Search

Navigation