Skip to main content
SpringerLink
Log in

Enhancement effect of cell adhesion on titanium surface using phosphonated low-molecular-weight chitosan derivative

  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

For improving cell adhesion on titanium surface, phosphonated low-molecular-weight chitosan (P-LMC) was prepared by introducing a phosphonate group of 4-phosphonobutyric acid to low-molecular-weight chitosan (3,000<MW<10,000). The phosphonate group of P-LMC was identified by 31P NMR. At about 30 ppm from external standard, there was a specific peak derived from phosphonate group. X-Ray Photoelectron Spectroscopy (XPS) and water contact angle were carried out for characterization of titanium surface treated with P-LMC. From these results, P-LMC coated on titanium surface was remained on that in spite of washing and P-LMC made titanium surface more hydrophilic. The biocompatibility of P-LMC was demonstrated through a cytotoxicity test using the C2C12 cell line because P-LMC should have no cytotoxicity for applying to dental implant materials. As a result, P-LMC was biocompatible to use as coating material of titanium. Improvement of cell adhesion on titanium surface treated with P-LMC was observed by microscopy, and the number of attached cells was counted using a hemocytometer. Titanium surface coated with P-LMC showed improved cell adhesion for 12 h. These results demonstrate that P-LMC is biocompatible and titanium surface treated with P-LMC has positive effects on cell adhesion. Therefore, P-LMC is a useful material that can be applied for titanium coating to enhance cell adhesion and osseointegration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. Hayakawa, Y. Masuda, K. Okamoto, Y. Shirosaki, K. Kato, and A. Osaka, J. Mater. Sci. Mater. Med., 25, 375 (2014).

    Article  CAS  Google Scholar 

  2. X. Liu, P. K. Chu, and C. Ding, Mater. Sci. Eng. R: Rep., 47, 49 (2004).

    Article  Google Scholar 

  3. E. Mohseni, E. Zalnezhad, and A. R. Bushroa, Int. J. Adhes. Adhes., 48, 238 (2014).

    Article  CAS  Google Scholar 

  4. D. Williams, in Titanium in Medicine, Springer, Heidelberg, 2001, pp 13–24.

    Book  Google Scholar 

  5. H. Zitter and H. Plenk, J. Biomed. Mater. Res., 21, 881 (1987).

    Article  CAS  Google Scholar 

  6. R. Strietzel, A. Hösch, H. Kalbfleisch, and D. Buch, Biomaterials, 19, 1495 (1998).

    Article  CAS  Google Scholar 

  7. B. Kasemo and J. Gold, Adv. Dent. Res., 13, 8 (1999).

    Article  CAS  Google Scholar 

  8. R. Fraioli, F. Rechenmacher, S. Neubauer, J. M. Manero, J. Gil, H. Kessler, and C. Mas-Moruno, Colloids Surf. B: Biointerfaces, 128, 191 (2015).

    Article  CAS  Google Scholar 

  9. H. Oonishi, M. Yamamoto, H. Ishimaru, E. Tsuji, S. Kushitani, M. Aono, and Y. Ukon, J. Bone Joint Surg. Br., 71-B, 213 (1989).

    Google Scholar 

  10. H.-M. Kim, F. Miyaji, T. Kokubo, and T. Nakamura, J. Biomed. Mater. Res., 32, 409 (1996).

    Article  CAS  Google Scholar 

  11. L. Le Guéhennec, A. Soueidan, P. Layrolle, and Y. Amouriq, Dental Materials, 23, 844 (2007).

    Article  Google Scholar 

  12. P. R. Klokkevold, R. D. Nishimura, M. Adachi, and A. Caputo, Clin. Oral Implants Res., 8, 442 (1997).

    Article  CAS  Google Scholar 

  13. X. Lu, Z. Zhao, and Y. Leng, Mater. Sci. Eng. C, 27, 700 (2007).

    Article  CAS  Google Scholar 

  14. B. Nasiri-Tabrizi and A. Fahami, J. Ind. Eng. Chem., 20, 1236 (2014).

    Article  CAS  Google Scholar 

  15. M. Golub, D. Lott, E. Watkins, V. Garamus, B. Luthringer, M. Stoermer, A. Schreyer, and R. Willumeit, Biointerphases, 8, 21 (2013).

    Article  Google Scholar 

  16. J. Amalric, P. H. Mutin, G. Guerrero, A. Ponche, A. Sotto, and J.-P. Lavigne, J. Mater. Chem., 19, 141 (2009).

    Article  CAS  Google Scholar 

  17. C.-M. Tîlmaciu, M. Mathieu, J.-P. Lavigne, K. Toupet, G. Guerrero, A. Ponche, J. Amalric, D. Noël, and P. H. Mutin, Acta Biomater., 15, 266 (2015).

    Article  Google Scholar 

  18. H. Lee, J. Rho, and P. B. Messersmith, Adv. Mater., 21, 431 (2009).

    Article  CAS  Google Scholar 

  19. I.-H. Bae, I.-K. Park, D. Park, H. Lee, and M. Jeong, J. Mater. Sci., Mater. Med., 23, 1259 (2012).

    Article  CAS  Google Scholar 

  20. G. Altankov and T. Groth, J. Mater. Sci., Mater. Med., 5, 732 (1994).

    Article  CAS  Google Scholar 

  21. A. Curtis, J. Forrester, C. McInnes, and F. Lawrie, J. Cell Biol., 97, 1500 (1983).

    Article  CAS  Google Scholar 

  22. F. Schwarz, M. Sager, D. Ferrari, M. Herten, M. Wieland, and J. Becker, J. Clin. Periodontol., 35, 64 (2008).

    Article  Google Scholar 

  23. S. Anil, H. Alghamdi, J. Jansen, and P. Anand, Dental Implant Surface Enhancement and Osseointegration, InTech Open Access Publisher, 2011.

    Book  Google Scholar 

  24. H.-J. Lee, S.-H. Park, S.-Y. Seo, Y.-M. Cho, H. Woo, Y. Ito, and T.-I. Son, Macromol. Res., 21, 216 (2013).

    Article  Google Scholar 

  25. S.-H. Park, S.-Y. Seo, H.-N. Na, K.-I. Kim, J.-W. Lee, H.-D. Woo, J.-H. Lee, H.-K. Seok, J.-G. Lee, S.-I. Chung, K. Chung, D. Han, Y. Ito, E.-C. Jang, and T.-I. Son, Macromol. Res., 19, 921 (2011).

    Article  CAS  Google Scholar 

  26. Y. Heo, H.-J. Lee, E.-H. Kim, M.-K. Kim, Y. Ito, and T.-I. Son, J. Appl. Polym. Sci., 131, n/a (2014).

  27. S. Y. Seo, S. H. Park, H. J. Lee, Y. Heo, H. N. Na, K. I. Kim, J. H. Han, Y. Ito, and T. I. Son, J. Appl. Polym. Sci., 128, 4322 (2013).

    Article  CAS  Google Scholar 

  28. Y.-M. Cho, H.-J. Lee, Y. Heo, S.-H. Park, S.-Y. Seo, J.-H. Han, and T.-I. Son, J. Appl. Polym. Sci., 129, 2161 (2013).

    Article  CAS  Google Scholar 

  29. M. Sadeghi-Kiakhani, M. Arami, and K. Gharanjig, J. Environ. Chem. Eng., 1, 406 (2013).

    Article  CAS  Google Scholar 

  30. M. Rinaudo, Prog. Polym. Sci., 31, 603 (2006).

    Article  CAS  Google Scholar 

  31. K.-I. Kim, J.-W. Lee, Y. Ito, J.-H. Kang, K.-S. Song, E.-C. Jang, and T.-I. Son, J. Appl. Polym. Sci., 117, 3029 (2010).

    CAS  Google Scholar 

  32. S.-H. Park, L. Zhu, S. Tada, S. Obuse, Y. Yoshida, M. Nakamura, T. I. Son, S. Tsuneda, and Y. Ito, Polym. Int., 63, 1616 (2014).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Systems Biotechnology and Bio-Environmental Technology (BET) Research Institute, Chung-Ang University, Anseong, Gyeonggi, 456-756, Korea

    Eun-Hye Kim, So-Yoon Chi, Hee-Dong Woo, Yun Heo & Tae-Il Son

  2. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan

    Shin-Hye Park & Yoshihiro Ito

  3. Center for Biomaterials, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul, 136-791, Korea

    So-Yoon Chi & Dong-Keun Han

  4. Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam, 540-950, Korea

    Jae-Woon Nah

Authors
  1. Eun-Hye Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shin-Hye Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. So-Yoon Chi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hee-Dong Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yun Heo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yoshihiro Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dong-Keun Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jae-Woon Nah
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Tae-Il Son
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tae-Il Son.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, EH., Park, SH., Chi, SY. et al. Enhancement effect of cell adhesion on titanium surface using phosphonated low-molecular-weight chitosan derivative. Macromol. Res. 24, 99–103 (2016). https://doi.org/10.1007/s13233-015-3135-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-015-3135-9

Keywords

Search

Navigation