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Diversity of fungi associated with roots of Eucalyptus gomphocephala seedlings grown in soil from healthy and declining sites

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Abstract

We used molecular profiling with 454 pyrosequencing to identify ectomycorrhizal, arbuscular mycorrhizal fungi, and other fungal communities associated with containerized Eucalyptus gomphocephala seedling roots. We found a higher proportion of ectomycorrizal fungi associated with seedling roots grown in soil collected from sites with healthy trees, and of arbuscular mycorrhizal fungi from seedling roots grown in soil collected from sites with declining trees. We also found a relatively high proportion of pathogenic fungi present in roots from declining sites compared to healthy sites. This research should be extended to the field to further investigate mycorrhizal and pathogenic fungal populations associated with tree declines.

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Acknowledgements

This work was supported by grants from the Australian Research Council and Murdoch University. We are grateful to Diana White for DNA preparation and to Assoc. Professor Treena Burgess for advice on molecular analysis.

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Correspondence to Giles E. St. J. Hardy.

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Ishaq, L., Barber, P.A., Hardy, G.E.S.J. et al. Diversity of fungi associated with roots of Eucalyptus gomphocephala seedlings grown in soil from healthy and declining sites. Australasian Plant Pathol. 47, 155–162 (2018). https://doi.org/10.1007/s13313-018-0548-x

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