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Induced degradation of crude oil mediated by microbial augmentation and bulking agents

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Abstract

Different bacterial and fungal strains, isolated from petroleum hydrocarbon-contaminated soil, were tested, in isolation as well as in combination, for their ability to degrade total petroleum hydrocarbon (TPH) in soil samples spiked with crude oil (2, 5 or 10 %, w/w) for 30 days. The selected combination of bacterial and fungal isolates, i.e., Pseudomonas stutzeri BP10 and Aspergillus niger PS9, exhibited the highest efficiency of TPH degradation (46.7 %) in soil spiked with 2 % crude oil under control condition. Further, when this combination was applied under natural condition in soil spiked with 2 % (w/w) crude oil along with inorganic fertilizers (NPK) and different bulking agents such as rice husk, sugarcane, vermicompost or coconut coir, the percent degradation of TPH was found to be maximum (82.3 %) due to the presence of inorganic fertilizers and rice husk as bulking agent. Further, results showed that the presence of NPK and bulking agents induced the activity of degradative enzymes, such as catalase (0.718 m mol H2O2 g−1), laccase (0.77 µmol g−1), dehydrogenase (37.5 µg g−1 h−1), catechol 1, 2 dioxygenase (276.11 µ mol g−1) and catechol 2, 3 dioxygenase (15.15 µ mol g−1) as compared to control (without bioaugmentation). It was inferred that the selected combination microbes along with biostimulants could accentuate the crude oil degradation as evident from the biostimulant-induced enhanced activity of degradative enzymes.

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References

  • Agbor RB, Ekpo IA, Osuagwa AN, Udofia UV, Okpako EC, Antai SP (2012) Biostimulation of microbial degradation of crude oil polluted soil using cocoa pod husk and plantain peels. J Microbiol Biotechnol Res 2(3):464–469

    CAS  Google Scholar 

  • Amenaghawon AN, Osunbor O, Obahiagbon KO (2014) Impact of nutrients, aeration and agitation on the bioremediation of crude oil polluted water using mixed microbial culture. Int J Sci Res Environ Sci 2(2):43–48

    Google Scholar 

  • Bahrampour T, Moghanlo V (2012) Evaluation of soil biological activity after soil contaminating by crude oil. Int J Agri Res Rev 2(6):671–679

    CAS  Google Scholar 

  • Beers JRF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140

    CAS  Google Scholar 

  • Beškoski VP, Gojgić-Cvijović GĐ, Milić JS, Ilić MV, Miletić Srđan B, Jovančićević BS, Vrvić-Miroslav M (2012) Bioremediation of soil polluted with crude oil and its derivatives: microorganisms, degradation pathways, technologies. Hemijska Ind 66(2):275–289

    Article  Google Scholar 

  • Binazadeh M, Karimi IA, Li Z (2009) Fast biodegradation of long chain n-alkanes and crude oil at high concentrations with Rhodococcus sp. Moj-3449. Enzyme Microbiol Technol 45(3):195–202

    Article  CAS  Google Scholar 

  • Boopathy R (2000) Factors limiting bioremediation technologies. Bioresour Technol 74:63–67

    Article  CAS  Google Scholar 

  • Chromo M, Sharifi HS, Motamedi H (2010) Bioremediation of a crude oil–polluted soil by applying of fertilizers. Iran J Environ Health Sci Eng 7(4):319–326

    Google Scholar 

  • Das TT, Gohel HR, Panchal MR, Ghosh SK, Braganza VJ (2014) Determination of crude oil degradation efficiency of glass biofilm of isolated bacterium and fungus. Int Res J Biol Sci 3(2):67–69

    Google Scholar 

  • Gilman JC (1990) A manual of soil fungi. Daya Publishing House, New Delhi

    Google Scholar 

  • Hamzah A, Salleh SN, Md Lee SL, Sarmani S (2012) Bioaugmentation of microbial consortia and supplementation of bulking agents in removal of crude oil from soil. In: Mendez-Vila A (ed) Microbes in applied research-current advances and challenges. Malaga, Spain, pp 39–43

    Chapter  Google Scholar 

  • Hazaimeh M, Mutalib SA, Abdullah PS, Kee WK, Surif S (2014) Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch. Ann Microbiol. doi:10.1007/s13213-014-0821-3

    Google Scholar 

  • Hegeman GD (1966) Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of the enzymes in the wild type. J Bacteriol 91:1140–1154

    CAS  Google Scholar 

  • Hollaway SL, Faw GM, Sizemore RK (1980) The bacterial community composition of an active oil field in the Northwestern Gulf of Mexico. Mar Pollut Bull 11(6):153–156

    Article  Google Scholar 

  • Huesemann MH, Hausmann TS, Fortman TJ (2003) Assessment of bioavailability limitations during slurry biodegradation of petroleum hydrocarbons in aged soil. Ecol Toxicol Chem 22(12):2853–2860

    Article  CAS  Google Scholar 

  • IEO (2014) International Energy Outlook DOE/EIA-0484. http://www.eia.gov/forecasts/ieo/pdf/0484%282014%29.pdf

  • Jin S, Fallgren PH (2007) Site-specific limitations of using urea as nitrogen source in biodegradation of petroleum wastes. Soil Sediment Contaminat 16(5):497–505

    Article  CAS  Google Scholar 

  • Jørgensen KS, Puustinen J, Suortti AM (2000) Bioremediation of petroleum hydrocarbon-contaminated soil by composting in biopiles. Environ Pollut 107:245–254

    Article  Google Scholar 

  • Kadali KK, Simons KL, Sheppard PJ, Ball AS (2012) Mineralization of weathered crude oil by a hydrocarbonplastic consortia in marine microcosms. Water Air Soil Pollut 223:4283–4295

    Article  CAS  Google Scholar 

  • Khashayar T, Mahsa T (2010) Biodegradation potential of petroleum hydrocarbons by bacterial diversity in soil. World Appl Sci J 8(6):750–755

    CAS  Google Scholar 

  • Klecka GM, Gibson DT (1981) Inhibition of catechol 2, 3-dioxygenase from Pseudomonas putida by 3-chlorocatechol. Appl Environ Microbiol 41(5):1159–1165

    CAS  Google Scholar 

  • Kumari B, Singh SN, Singh DP (2012) Characterization of two biosurfactant producing strains in crude oil degradation. Process Biochem 47(12):2463–2471

    Article  CAS  Google Scholar 

  • Kumari B, Singh SN, Farah D, Sharma M, Pandey V, Singh DP (2013) Elucidation of pyrene degradation pathway in bacteria. Adv Bioresour 4(2):151–160

    CAS  Google Scholar 

  • Lavania M, Cheema S, Sarma PM, Mandal AK, Lal B (2012) Biodegradation of asphalt by Garciaella petrolearia TERIG02 for viscosity reduction of heavy oil. Biodegrad 23:15–24

    Article  CAS  Google Scholar 

  • Li L, Cunningham CJ, Pas V, Philp JC, Barry DA, Anderson P (2004) Field trial of a new aeration system for enhancing biodegradation in a biopile. Waste Manag 24:127–137

    Article  CAS  Google Scholar 

  • Liu PWG, Wang SY, Huang SG, Wang MZ (2012) Effects of soil organic matter and ageing on remediation of diesel contaminated soil. Environ Technol 33(23):2661–2672

    Article  CAS  Google Scholar 

  • Margesin R, Zimmerbauer A, Schinner F (2004) Monitoring of bioremediation by soil biological activities. Chemosphere 40:339–346

    Article  Google Scholar 

  • Mishra S, Jyot J, Kuhad RC, Lal B (2001) In situ bioremediation potential of an oily sludge-degrading bacterial consortium. Curr Microbiol 43:328–335

    Article  CAS  Google Scholar 

  • Mortazavi B, Horel A, Beazley MJ, Sobecky PA (2013) Intrinsic rates of petroleum hydrocarbon biodegradation in gulf of Mexico inter tidy sandy sediments and its enhancement by organic substrates. J Hazard Mater 244–45:537–544

    Article  Google Scholar 

  • Prince R, McMillen S (2002) Summary of PERF bioremediation projects. In: Presentation at DOE/PERF bioremediation workshop. May 30 Houston

  • Rahman KSM, Thahira-Rahman J, Lakshmanperumalsamy P, Banat IM (2002) Towards efficient crude oil degradation by a mixed bacterial consortium. Bioresour Technol 85:257–261

    Article  CAS  Google Scholar 

  • Rizi MS, Sepahi AA, Tabatabaee MS (2012) Crude oil biodegrdation by a soil indigenous Bacillus sp. isolated from Lavan Island. Biorem J 16(4):218–224

    Article  CAS  Google Scholar 

  • Salmon C, Crabos JL, Sambuco JP, Bessiere JM, Basseres A, Caumette P, Baccou JC (1998) Artificial wetland performances in the purification efficiency of hydrocarbon wastewater. Water Air Soil Pollut 104:313–329

    Article  CAS  Google Scholar 

  • Sampath R, Venkatakrishnan H, Ravichandran V, Chaudhury RR (2012) Biochemistry of TBT degrading marine Pseudomonas isolated from Indian coastal water. Water Air Soil Pollut 223:99–106

    Article  CAS  Google Scholar 

  • Satishkumar M, Binupriya AR, Balk SH, Yun SE (2008) Biodegradation of crude oil by individual bacterial strains and mixed bacterial consortium isolated from hydrocarbon contaminated areas. Clean 36(1):92–96

    Google Scholar 

  • Shahaby AF (2014) Assessment mixed culture of Actinomyces and Sacchromyces for biodegradation of complex mineral oil hydrocarbon. Int J Curr Microbiol App Sci 3(4):401–414

    Google Scholar 

  • Stevenson IL (1956) Some observations on the microbial activity in remoistened air-dried soils. Plant Soil 8(2):170–182

    Article  Google Scholar 

  • Trémier A, Telia C (2009) Barrington S. Effect of initial physical characteristics on sludge compost performance. Bioresour Technol 100:3751–3758

    Article  Google Scholar 

  • Vincent AO, Felix E, Weltime MO, Ize-iyamu OK, Daniel EE (2011) Microbial degradation and its kinetics on crude oil polluted soil. Res J Chem Sci 1(6):8–14

    CAS  Google Scholar 

  • Wu M, Chen L, Tian Y, Ding Y, Dick WA (2013) Degradation of polycyclic aromatic hydrocarbons by microbial consortia enriched from three soils using two different culture media. Environ Pollut 178:152–158

    Article  CAS  Google Scholar 

  • Xu YH, Lu M (2010) Bioremediation of crude oil-contaminated soil: comparison of different biostimulation and bioaugmentation treatments. J Hazard Mater 183(1–3):395–401

    Article  CAS  Google Scholar 

  • Yakubu BM, Ma H, Zhang CY (2009) Biodegradation of crude oil in soil using chicken manure. Int J Environ Pollut 36:400–410

    Article  CAS  Google Scholar 

  • Yan S, Wan Q, Qu L, Li C (2013) Characterization of oil- degrading bacteria from oil-contaminated soil and activity of their enzymes. Biotechnol Biotechnol EQ.27/2013/4, 3932–3938

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Acknowledgments

Authors are thankful to Director, CSIR-NBRI, UP (India), for providing the facilities to execute this study and CSIR HQ for financial support to the project NWP019. Authors also acknowledge Dr. Dilip Kumar, CSIR-NIEST, Jorhat, West Bengal (India), for providing us the bacterial strain NJ2 which was used in this investigation.

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Authors and Affiliations

  1. Environmental Science Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow, UP, 226001, India

    B. Kumari & S. N. Singh

  2. School of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow, India

    D. P. Singh

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  1. B. Kumari
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  2. S. N. Singh
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Correspondence to S. N. Singh.

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Kumari, B., Singh, S.N. & Singh, D.P. Induced degradation of crude oil mediated by microbial augmentation and bulking agents. Int. J. Environ. Sci. Technol. 13, 1029–1042 (2016). https://doi.org/10.1007/s13762-016-0934-2

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  • DOI: https://doi.org/10.1007/s13762-016-0934-2

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