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High genetic variability in self-incompatible myophilous Octomeria (Orchidaceae, Pleurothallidinae) species

  • Reproductive Biology
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Abstract

Octomeria crassifolia Lindl. and O. grandiflora Lindl. are myophilous, self-incompatible and partially inter-compatible species. In order to better understand the relationships between their reproductive systems, patterns of genetic variability, and isolation mechanisms in sympatric populations, a genetic study using ISSR molecular markers was carried out in natural populations growing in southeastern Brazil. The populations of both species demonstrated moderately high genetic variability greater than that observed in other self-compatible melittophilous orchid species, indicating that self-incompatibility may be a determinant factor in maintaining higher variability levels in myophilous Pleurothallidinae species. Contrary to what might be expected due to behavior of their pollinators of flying short distances, these species of Octomeria demonstrated relatively low genetic structuring that was probably related to gene flow by seeds or to older shared genetic stock. Bayesian analysis of genetic structuring indicated the presence of three genetic groups in O. crassifolia (although without any relation to their geographical distributions) and two genetic groups in O. grandiflora (with one of them restricted to one of the populations). No indications were seen of hybridization or introgression among the sympatric populations, indicating that pollinator specificity is an important factor in guaranteeing the identities of these inter-compatible species.

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Acknowledgments

We thank Marcos C. de Melo and Pedro P. G. Taucce for help with population sampling, and Júnia M. Lousada for help in some laboratory tasks. This work was funded by projects from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil. ARB received a fellowship from CNPq. ELB is supported by a productivity grant from CNPq.

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Authors and Affiliations

  1. Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil

    Ariane Raquel Barbosa

  2. Setor de Ciências Biológicas, Departamento de Botânica, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, PR, 81531-990, Brazil

    Viviane Silva-Pereira

  3. Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Santa Adélia, 166, Bangu, Santo André, SP, 09210-170, Brazil

    Eduardo Leite Borba

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  1. Ariane Raquel Barbosa
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  2. Viviane Silva-Pereira
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  3. Eduardo Leite Borba
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Correspondence to Eduardo Leite Borba.

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Barbosa, A.R., Silva-Pereira, V. & Borba, E.L. High genetic variability in self-incompatible myophilous Octomeria (Orchidaceae, Pleurothallidinae) species. Braz. J. Bot 36, 179–187 (2013). https://doi.org/10.1007/s40415-013-0027-0

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