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Preparation and characterization of electrospun polylactic acid/sodium alginate/orange oyster shell composite nanofiber for biomedical application

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Abstract

Bone tissue engineering has begun to draw attention in recent years. The interactive combination of biomaterials and cells is part of bone tissue engineering. Sodium alginate (SA) is a biologically compatible, degradable, non-toxic natural polymer accepted by the human body and is widely used in the field of tissue engineering. Polylactic acid (PLA) is another type of biodegradable thermoplastic polyester derived from renewable sources which are used in bone tissue engineering and biomedical owing to its biocompatibility and biodegradability. Hydroxyapatite (HA) and tricalcium phosphate (TCP) derived from natural sources such as marine species and bovine bone are biocompatible and non-toxic biomaterials which are used to reconstruct many parts of the skeleton. In this study, PLA, SA with different compositions, and nanofibers obtained by adding orange spiny oyster shell powders (Spondylus barbatus) to them by using electrospining technique. Cell culture study, scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and physical analysis such as density, electrical conductivity, surface tension, viscosity measurement, and tensile strength measurement tests were carried out after the production process. Produced nanofibers showed smooth and beadless surface. The average diameters and distributions decreased with the addition of optimum PLA and TCP amount. The tensile strength of nanofibers was enhanced with the additional SA and TCP. The produced nanofibers are compatible with human bone tissue, which are not cytotoxic, and in addition, a high cell efficiency of SaOS-2 cells on the nanofibers was observed with SEM images.

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Funding

This study has been funded by the Ministry of Development, Turkey; project no: 2016K121280.

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Authors and Affiliations

  1. Center for Nanotechnology & Biomaterials Application and Research (NBUAM) at Marmara University and Faculty of Technology, Department of Metallurgical and Materials Engineering, Marmara University, 34722, Istanbul, Turkey

    Sumeyye Cesur & Oguzhan Gunduz

  2. Center for NBUAM and Department of Bioengineering, Faculty of Engineering, Marmara University, 34722, Istanbul, Turkey

    Faik Nuzhet Oktar

  3. Center for NBUAM and Department of Electrical and Electronic Engineering, Faculty of Technology, Marmara University, 34722, Istanbul, Turkey

    Nazmi Ekren

  4. Center for NBUAM and Department of Electrical and Electronics Engineering, Faculty of Engineering, Marmara University, 34722, Istanbul, Turkey

    Osman Kilic

  5. Center for NBUAM and Department of Analytical Chemistry, Faculty of Pharmacy, Marmara University, 34668, Istanbul, Turkey

    Dilek Bilgic Alkaya & Serap Ayaz Seyhan

  6. Center for NBUAM and Department Biomedical Engineering, Faculty of Engineering, Istanbul University, 34320, Istanbul, Turkey

    Zeynep Ruya Ege

  7. Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan

    Chi-Chang Lin

  8. School of Medicine and Department of Physiology, Istanbul University, 34390, Istanbul, Turkey

    Serap Erdem Kuruca

  9. Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, 34390, Istanbul, Turkey

    Gokce Erdemir

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  1. Sumeyye Cesur
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Cesur, S., Oktar, F.N., Ekren, N. et al. Preparation and characterization of electrospun polylactic acid/sodium alginate/orange oyster shell composite nanofiber for biomedical application. J Aust Ceram Soc 56, 533–543 (2020). https://doi.org/10.1007/s41779-019-00363-1

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