Parinaric acid as a sensitive fluorescent probe for the determination of lipid peroxidation

doi:10.1016/0005-2760(87)90027-0

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism

Volume 921, Issue 2, 25 September 1987, Pages 266-274

Biochimica et Biophy...

https://doi.org/10.1016/0005-2760(87)90027-0 Get rights and content

Abstract

The decrease in fluorescence of conjugated polyenic acyl chains is used as a sensitive assay for lipid peroxidation. The fatty acid cis-trans-trans-cis-9,11,13,15-octadecatetraenoic acid (cis-parinaric acid) is introduced into liposomal membranes as free fatty acid or, by using the PC specific transfer protein from bovine liver, as 1-palmitoyl-2-cis-parinaroyl-sn-glycero-3-phosphocholine. The peroxidation process as monitored by the decrease in fluorescence intensity is compared with other peroxidation assay systems. Applications of the new assay system are discussed.

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Citation Excerpt :
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Parinaric acid as a sensitive fluorescent probe for the determination of lipid peroxidation

Abstract

Methods Enzymol.

Chem. Phys. Lipids

Chem. Phys. Lipids

J. Lipid Res.

Biochim. Biophys. Acta

Methods Enzymol.

J. Lipid Res.

J. Lipid Res.

Biochim. Biophys. Acta

Anal. Biochem.