Demonstration of a glycoprotein which is associated with a purified myelin fraction from rat brain

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Abstract

Rats were injected intracerebrally with radioactive fucose in order to label specifically the glycoproteins of brain. The radioactive glycoproteins were fractionated by polyacrylamide electrophoresis in the presence of sodium dodecyl sulfate. The purified myelin fraction contained a major radioactive glycoprotein with an apparent molecular weight of ∼110,000. The glycoprotein could also be stained with periodic acid-Schiff reagent.

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