Covalent immobilization of proteins on to the surface of poly(vinyl alcohol) hydrogel
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Effects of a dielectric barrier discharge (DBD) treatment on chitosan/polyethylene oxide nanofibers and their cellular interactions
2018, Carbohydrate PolymersCitation Excerpt :Chitosan (CS), a natural polymer obtained by alkaline deacetylation of chitin, is a linear polysaccharide consisting of glucosamine and N-acetylglucosamine units. Its molecular structure is rich in hydroxyl (OH) and amino (NH2) groups, both being attractive options for grafting of bioactive compounds (Holländer, Pippig, Dubreuil, & Vangeneugden, 2008; Kobayashi & Ikada, 1991; Ruiz et al., 2014; Wong & Abdul-Aziz, 2008). The biocompatibility, biodegradability, bioactivity, adhesiveness and non-toxicity of CS, together with its specific interactions with components of ECM and growth factors, make it a relevant candidate for tissue engineering applications, such as skin repair, bone regeneration, cartilage repair, nerve conduit fabrication, contact lens manufacturing and for use as surgical sutures and as wound dressing material (Ko, Kawazoe, Tateishi, & Chen, 2010; Rafique, Zia, Zuber, Tabasum, & Rehman, 2016; Uddin, Araki, & Gotoh, 2011).
Recent developments in L-glutaminase production and applications – An overview
2017, Bioresource TechnologyCitation Excerpt :There are many advantages for the immobilization of cell and enzyme, such as reduce the time for isolation and purification of enzyme, cut down the cost of the enzymes since the immobilized enzymes can be reused for many reaction cycle, stability of enzyme is increased towards the environmental conditions such as pH, temperature and ionic strength, reduces the volume of bioreactors and other benefits. Enzymes have large number of practical applications such as biomaterials (Kobayashi and Ikada, 1991) and biosensors (Karakuş et al., 2013; Yılmaz and Karakus, 2011), through immobilization on a variety of supports. Beside their specificity, the short catalytic life times of enzymes presently limit their usefulness.
Natural polysaccharides promote chondrocyte adhesion and proliferation on magnetic nanoparticle/PVA composite hydrogels
2015, Colloids and Surfaces B: BiointerfacesCitation Excerpt :However, its intrinsically cell non-adhesive nature provides poor support to cell growth and integration to peripheral tissues [14,15]. Modification of PVA with biomolecules such as arginine–glycine–aspartic acid (RGD) peptide [16], hydroxyapatite [17] or natural polysaccharide (chondroitin sulfate) has shown improvements in cell adhesion and growth [18]. For example, hydroxyapatite (HAP) is the main inorganic composition in bone and has good osteoconductivity.
Regenerative approaches as alternatives to donor allografting for restoration of corneal function
2012, Ocular SurfaceCitation Excerpt :In both cases, however, extracellular matrix macromolecules and/or growth factors or derivatives are often applied as surface coatings to promote regeneration. Naturally occurring extracellular matrix proteins, such as collagen, fibronectin, and laminin, and derivative cell adhesive peptides such as IKVAV, YIGSR and RGD, have been grafted onto the KPro's,17,18,23-25 although other factors including pore size and surface topography26 can also impact device epithelization. Although results have not been entirely confirmed in an in vivo model, in vitro work suggests that corneal epithelial cell growth and adhesion were significantly enhanced by tethering of laminin or fibronectin adhesion-promoting peptide (FAP) via flexible poly(ethylene) glycol (PEG) chains, more so than by tethering of fibronectin or simple coating of the surface with matrix proteins.17,18
Engineering the corneal epithelial cell response to materials
2009, Biomaterials and Regenerative Medicine in Ophthalmology