Light inducible and tissue-specific expression of a chimeric mouse metallothionein cDNA gene in tobacco

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Abstract

A metallothionein cDNA was placed in sense or antisense orientation under the transcriptional control of a light-regulated promoter and the 3′ flanking region from a pea ribulose bisphosphate carboxylase small subunit gene and introduced into Nicotiana tabacum by Agrobacterium-mediated transformation. Regenerated plants with the sense construct showed expression of metallothionein (MT) in a light-regulated, tissue-specific manner. Genetic analysis of R1 and R2 progeny showed inheritance of the kanamycin resistance marker gene as a dominant Mendelian trait. Transgenic young seedlings with rbcS(ribulose bisphosphate carboxylase small subunit)-MT construct showed slightly more tolerance to cadmium stress than did untransformed control seedlings. In addition to these studies, a comparison was made of the relative strengths of the CaMV 35S, rbcS, and mouse metallothionein promoters in plants. The MT promoter was virtually inactive in plants, even those treated with Cd.

References (38)

  • J.J. Fillatto et al.

    Efficient transfer of a glyphosphate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector

    Bio-Technology

    (1987)
  • M. Vaeck et al.

    Transgenic plants protected from insect attack

    Nature

    (1987)
  • D.A. Fischhoff et al.

    Insect tolerant transgenic tomato plants

    Bio-Technology

    (1987)
  • K.A. Barton et al.

    Bacillus thuringiensis δ-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to Lepidopteran insects

    Plant Physiol.

    (1987)
  • P. Powell-Abel et al.

    Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene

    Science

    (1986)
  • B.D. Harrison et al.

    Virus resistance in transgenic plants that express cucumber mosaic virus satellite RNA

    Nature

    (1987)
  • W.L. Gerlach et al.

    Construction of a plant disease resistance gene from the satellite RNA of tobacco ringspot virus

    Nature

    (1987)
  • S. McCormick et al.

    Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens

    Plant Cell Rep.

    (1986)
  • M.A.W. Hinchee et al.

    Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer

    Bio-Technology

    (1988)
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