The l-arabinose permease system in Escherichia coli B/r

doi:10.1016/0304-4165(66)90169-3

Biochimica et Biophysica Acta (BBA) - General Subjects

Volume 117, Issue 1, 28 March 1966, Pages 217-230

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Abstract

The l-arabinose permease system of Escherichia coli B/r was characterized by measuring l-[i-^14C]arabinose uptake by an l-arabinose isomerase-less mutant. This organism accumulates l-arabinose via an inducible, energy-dependent system that exhibits the kinetic properties of permease-mediated transport. The system consists of at least 2 parts: an entrance reaction that obeys Michealis-Menten kinetics and an exit reaction with a high temperature coefficient that exhibits first-order kinetics. Inhibition studies indicate that the system may also mediate the transport of d-xylose, d-frucose, and d-galactose. A “cold shock” phenomenon which results in a rapid loss in accumulated carbohydrates is described.

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^∗: Present address: Department of Biological Sciences, University of California, Santa Barbara, Calif., U.S.A.

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The l-arabinose permease system in Escherichia coli B/r

Abstract

Biochim. Biophys. Acta

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Virology

J. Biol. Chem.

Biochim. Biophys. Acta