Bacterial cell disruption: A key unit operation in the recovery of intracellular products

doi:10.1016/0734-9750(91)90005-G

Biotechnology Advances

Volume 9, Issue 2, 1991, Pages 217-240

https://doi.org/10.1016/0734-9750(91)90005-G Get rights and content

Abstract

The need for microbial cell disruption has hindered the large scale production of commercial biotechnological products of intracellular derivation. The intracellular nature of many recombinant products and the potential use of the bacterial storage product, PHB as a commodity thermoplastic have renewed interest in the improvement of this unit operation. This paper provides a review of processes of a mechanical, physical, chemical or biological nature used for cell disruption on both the laboratory and large scale. Applicability of the techniques to large scale operation is discussed. Modification of existing processes is suggested for the reduction of energy requirements and improved process economics. The requirements for the liberation of granular intracellular products such as inclusion bodies and virus-like yeast particles are distinguished from those for the liberation of soluble products, mainly proteinaceous in nature. The integrated nature of the process with both upstream and downstream processes is addressed. Finally, the recent approach of selective liberation of soluble products of interest is reviewed.

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¹: Present Address: Department of Chemical Engineering, University of Cape Town, Private Bag, Rondebosch 7700, South Africa

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Research review paperBacterial cell disruption: A key unit operation in the recovery of intracellular products

Abstract

Tibtech.

J. Microbiol. Methods

Biotechnol. Advs

Enz. Microb. Technol.

Anal. Biochem.

Biochim. Biophys. Acta

Enz Microb. Technol.

Enz. Microb. Technol.

Enz. Microbial Technol.

Differential product release from yeast cells by selective enzymatic lysis

The isolation of lytic enzymes from Cytophaga and their application to the rupture of yeast cells

Biotechnol. Bioeng.

Disruption of Saccharomyces cerevisiae using enzymatic lysis combined with high pressure homogenisation

Biotechnology Techniques

Response of cell walls of Escherichia coli to a sudden reduction of the environmental osmotic pressure

J. Bacteriol.

Verfahren zur Gewinnung von Protein aus Mikroorganismen

Bioseparations: downstream processing for biotechnology

Simple and rapid method for the disruption of bacteria for protein studies

Appl. Environ. Microbiol.

Mechanism of cell disintegration in a high pressure homogeniser

Biotechnol. Bioeng.

Further studies on the mechanism of cell disruption by extreme pressure extrusion

Biotechnol. Bioeng.

Lysozyme sensitivity of the cell wall of Pseudomonas aeruginosa

Can. J. Microbiol.

The penicillin-binding proteins of Bacilli

The dependence of protein release from Bacillus amyloliquefaciens on the growth phase in batch culture

Biotechnol. Bioeng.

Release of protein from Baker's yeast (Saccharomyces cerevisiae) by disruption in an industrial agitator mill

Biotechnol. Bioeng.

The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins

Biotechnol. Bioeng.

Downstream process economics

Mechanism of disintegration of biological cells in ultrasonic cavitation

Biotechnol. Bioeng.

Lysis of cell walls and intact cells of Pseudomonas aeroginosa by EDTA and by lysozyme

J. Microbiol.

Novel shock-tube cell-disrupter

Process Biochemistry

Disruption of Candida utilis cells in high pressure flow devices

Biotechnol. Bioeng.

Disruption of microbial cells

Solubilisation of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate

J. Bact.

The interactions between fermentation and protein recovery

Bio/technology

Release of protein from Baker's yeast by disruption in an industrial homogeniser

Biotechnol. Bioeng.

Bursting bacteria by release of gas pressure

Nature

Biosynthesis of peptidoglycan

Lysis of Hydrogenomonas eutropha Z-1 cells by the enzymes of Micromonospora vulgaris PA II-4

Mikrobiologiya

The continuous-flow isolation of enzymes

Critical notes on the applicability of ultrasound to chemical technology

Int. Chem. Engng

Research review paper
Bacterial cell disruption: A key unit operation in the recovery of intracellular products