Noninvasive measures of oxidative stress status in humans

doi:10.1016/0891-5849(91)90073-C

Free Radical Biology and Medicine

Volume 10, Issues 3–4, 1991, Pages 177-184

https://doi.org/10.1016/0891-5849(91)90073-C Get rights and content

Abstract

Although oxidative stress is thought to be involved in the pathophysiology of several diseases and aging, it is not routinely measured in clinical diagnosis. This is at least partly because accepted and standardized methods for measuring oxidative stress in humans are not yet established. One of the greatest needs in the field of free radical biology is the development of reliable methods for measuring oxidative stress status (OSS) in humans. A listing of some analytical approaches to measuring oxidative stress is provided as well as a listing of some noninvasive techniques that have been used in humans.

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Thus, they are capable of binding to protein and nucleic acids and even impairing their functions [9]. Additionally, they are considered as potential marker for oxidative stress and metabolic status [10], which has been proposed to estimate cancer disease [11] and symptoms of alcohol abuse [12]. Hence, it is critical to establish a method with high selectivity and sensitivity for the determination of aldehydes in various samples.
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Substantial evidence shows the association between the level of these biomarkers and development of many diseases. Accordingly, the lipid peroxidation products have received much attention as biomarkers of oxidative stress and diseases and also as indicators of antioxidant effects and the advantages and limitations of various biomarkers and methods of measurement have been the subject of extensive studies and arguments [1–10]. Lipids are vulnerable to oxidation by both enzymes and non-enzymatic oxidants.
Free radical-mediated lipid peroxidation has been implicated in a number of human diseases. Diverse methods have been developed and applied to measure lipid peroxidation products as potential biomarkers to assess oxidative stress status in vivo, discover early indication of disease, diagnose progression of disease, and evaluate the effectiveness of drugs and antioxidants for treatment of disease and maintenance of health, respectively. However, standardized methods are not yet established.
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Lipid hydroxides such as hydroxyoctadecadienoic acids (HODE), hydroxyeicosatetraenoic acids (HETE), and hydroxycholesterols may be recommended as reliable biomarkers. Notably, the four HODEs, 9-cis,trans, 9-trans,trans, 13-cis,trans, and 13-trans,trans-HODE, can be measured separately by LC–MS/MS and the trans,trans-forms are specific marker of free radical mediated lipid peroxidation. Further, isoprostanes and neuroprostanes are useful biomarker of lipid peroxidation. It is important to examine the distribution and temporal change of these biomarkers.
Despite the fact that lipid peroxidation products are non-specific biomarkers, they will enable to assess oxidative stress status, disease state, and effects of drugs and antioxidants. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.

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^☆: Work in our laboratories on oxidative stress is supported in part by grants from NIH and a contract from the National Foundation for Cancer Research.

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Symposium in printNoninvasive measures of oxidative stress status in humans☆

Abstract

J. Free Radic. Biol. Med.

Mutat. Res.

J. Biol. Chem.

Free Radic. Biol. Med.

J. Biol. Chem.

J. Biol. Chem.

J. Lipid Res.

Anal. Biochem.

Anal. Biochem.

Trends in Biochem. Sciences

Anal. Biochem.

Methods in Enzymology

Biochem. Biophys. Res. Commun.

Chem.-Biol. Interact.

Chem.-Biol. Interact.

Free Radic. Biol. Med.

Biochim. Biophys. Acta

Free Radic. Biol. Med.

J. Lipid Res.

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Free Radic. Biol. Med.

Anal. Biochem.

Biochim. Biophys. Acta

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Chem. Phys. Lipids

Free Radic. Biol. Med.

J. Free Radic. Biol. Med.

Oxidants and human disease: some new concepts

FASEB J.

The role of free radical reactions in biological systems

Oxyradicals and related species: their formation, lifetimes, and reactions

Annu. Rev. Physiol.

Free radical theory of aging: the “free radical” diseases

Age

Symposium in print
Noninvasive measures of oxidative stress status in humans☆