Piezoelectric immuno sensor for the detection of candida albicans microbes

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Abstract

The system consists of an AT-cut quartz piezoelectric crystal, oscillator and frequency counter. The surface of the palladium-plated electrodes is oxidized anodically, and anti-Candida antibody is immobilized onto the surface. The crystal sensor is dipped into Candida suspension and the surface mass increase, caused by immuno-adsorption of Candida, is measured by the decrease in the resonant frequency of the crystal. The frequency shift is correlated with C. albicans concentrations in the range 106−5 × 108 cells cm−3. The crystal sensor showed no response to Saccharomyces cerevisiae.

References (15)

  • K.H. Karmarkar et al.

    Anal. Chim. Acta

    (1975)
  • T. Nomura et al.

    Anal. Chim. Acta

    (1981)
  • T. Nomura et al.

    Anal. Chim. Acta

    (1985)
  • T.J. Roger et al.

    Microb. Rev.

    (1980)
  • T.E. Kiehn

    Science

    (1979)
  • M.A. Persi et al.

    Appl. Environ. Microbiol.

    (1981)
  • H. Matsuoka et al.

    Denki Kagaku

    (1982)
There are more references available in the full text version of this article.

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