Occurrence of a cobalt-induced and cobalt-containing nitrile hydratase in RhodococcusRhodochrous J1

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Summary

The formation of nitrile hydratase required cobalt ions in Rhodococcusrhodochrous J1. No other transition-metals could replace the cobalt ion. The Rhodococcus nitrile hydratase was purified to homogeneity and found to contain a cobalt atom. The occurrence of a cobalt-induced and cobalt-containing nitrile hydratase, different from the nitrile hydratases in Pseudomonaschlororaphis B23 and Brevibacterium R312 containing a ferric ion in their active center, has been demonstrated here for the first time.

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