Elsevier

Carbohydrate Research

Volume 156, 15 November 1986, Pages 173-187
Carbohydrate Research

Identification and location of l-glycerate, an unusual acyl substituent in gellan gum

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Abstract

Gellan gum, the extracellular polysaccharide produced by Pseudomonas elodea, had been shown to be composed of a repeating, partially acetylated tetrasaccharide. Through the use of selective solvolysis of the rhamnosyl linkages in the polysaccharide with anhydrous, liquid hydrogen fluoride at −40°, oligosaccharides representing the repeating unit of the polymer in various acylated forms have now been generated. By a combination of n.m.r. spectroscopy, f.a.b.-mass spectrometry, and gas-liquid chromatographic analysis, these oligomers were shown to be substituted mainly with l-glyceric esters on O-2 of what was the 3-linked d-glucose in the original polymer, and substituted to a lesser degree with an acetic ester at O-6 of the same residue. The native gum must have the following structure.

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