Polyamines, Phospholipids, and Peroxides in Normal and Habituated Sugar Beet Calli

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Summary

Compared to a normal (N) auxin and cytokinin requiring sugar beet callus, the habituated (H) auxin and cytokinin independent callus of the same plant contained the same amount of total phospholipids during the course of culture.The peroxide indices of both calli were quite different with an about 2.5 higher level in the N callus.The H callus contained a higher amount of spermine and spermidine than the N one.The contents of putrescine were quite similar.The H callus was characterized by an about 100% increase of its spermine and spermidine content in the 3–14 days following subculture, just before and during active divisions in this highly meristematic tissue.It is hypothesized that the previously shown low level of ethylene production in the H callus might be due to the high amount of polyamines.These substances might also explain the low level of peroxides in the H callus.

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