Biomedical applications of maghemite ferrofluid

doi:10.1016/S0300-9084(00)80006-1

Biochimie

Volume 80, Issues 5–6, May–June 1998, Pages 379-390

https://doi.org/10.1016/S0300-9084(00)80006-1 Get rights and content

Abstract

The use of cell-targeted ferrofluid in the characterization of modifications of cell membranes is reviewed. Maghemite ferrofluid was synthesized by the Massart method, complexed with dimercaptosuccinic acid (FF). Cell targeting by FF was developed by coupling FF to various biological effectors such as antibodies, lectins, etc, which enabled magnetic cell sorting. Modifications in erythrocyte membranes were studied using FF bound to recombinant human annexin V (AnxFF) which is very sensitive, compared to other Anx-based reagents, in the early detection of phosphatidylserine (PS) exposition on the outer leaflet of the plasma membrane. Thus PS exposition on mouse RBC was detected already after a 24-h storage at 4°C and, transiently, 24 h after their infection by Plasmodium parasites, at which time the parasites are still confined to the liver, thus leading to the recruitment of young RBC and the accumulation of a species, intermediate between reticulocytes and erythrocytes, and the actual RBC target of plasmodial invasion. AnxFF revealed PS exposition on RBC from sickle cell anemia patients, following various inflammations and already after 20 days of human blood storage under blood bank conditionss.Such a sensitive detection should be similar to that of macrophages which recognize exposed PS on cells and bring about the latter's elimination from the circulation. AnxFF binding determination was combined with that of cell electrophoretic mobility, glycerol resistance and filterability to characterize RBC membrane modifications in Alzheimer's disease patients which suggested a continuous damage and regeneration in RBC of these patients. A logistic analysis suggested that several three-parameter combinations could permit diagnosis of Alzheimer's disease with up to 95% accuracy. THP1 cells and macrophages, derived themselves by incubation with retinoic acid, were bound to FF and placed in a radio frequency alternating magnetic field. Magnetocytolysis was associated with FF attachment to the cells without damage to non-bound cells and without heating of the surrounding solution.

References (81)

R.S. Molday et al.
Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells
J Immunol Methods
(1982)
P. Raynal et al.
Annexins: the problem of assessing the biological role for a gene family of multifucntional calcium- and phospholipid-binding proteins
Biochim Biophys Acta
(1994)
A.J. Schroit et al.
In vivo recognition and clearance of red blood cells containing phosphatidylserine in their plasma membrane
J Biol Chem
(1985)
G. Koopman et al.
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis
Blood
(1994)
F.A. Kuypers et al.
Detection of altered membrane phospho-lipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V
Blood
(1996)
B.L. Wood et al.
Increased erythrocyte phosphatidylserine exposure in sickle cell disease: Flow-cytometric measurement and clinical associations
Blood
(1996)
J. Taverne et al.
Malaria: Phosphatidylserine expression is not increased on the surface of parasitized erythrocytes
Parasitol Today
(1995)
A. Halbreich et al.
Annexin V binding to mouse erythrocytes following infection with Plasmodium parasites
Parasitol Today
(1996)
M.F. Folstein et al.
Mini Mental State: a practical method for grading the cognitive state of patients for the clinician
J Psychiatr Res
(1975)
R. Sulkava et al.
APOE alleles in Alzheimer's disease and vascular dementia in a population aged 85+
Neurobiol Aging
(1996)

P. Kehoe et al.

UK Alzheimer's Disease Collaborative Group. Confirmation of an association between a presenilin-1 intronic polymorphism and late onset Alzheimer's disease

Lancet

(1996)

A. Maelicke et al.

New approach to drug therapy in Alzheimer's dementia

Drug Discovery Today

(1996)

G.J. Bosman et al.

Erhthrocyte membrane changes of individuals with Down's syndrome in various stages of Alzheimer-type dementia

Neurobiol Aging

(1993)

G.J. Bosman et al.

Erythrocyte membrane characteristics indicate abnormal cellular aging in patients with Alzheimer's disease

Neurobiol Aging

(1991)

F. Shalit et al.

T lymphocyte subpopulations and activation markers correlate with severity of Alzheimer's disease

Clin Immunol Immunopathol

(1995)

M. Lejoyeux et al.

No change in viscosity of lipid phase of RBC membrane in Alzheimer's disease

Psychiatry Res

(1993)

H. Walter et al.

Red blood cells from Alzheimer patients and from normal subjects discerned by cell electrophoresis in an aqueous polymer solution

Biochem Biophys Res Commun

(1993)

H. Walter et al.

Differential electrophoretic behavior in aqueous polymer solutions of red blood cells from Alzheimer's patients and from normal individuals

Biochim Biophys Acta

(1995)

A. Sauer et al.

Kinetics of hemolysis of normal and abnormal red blood cells in glycerol-containing media

Biochim Biophys Acta

(1991)

K. Chandrasekaran et al.

Impairment in mitochondrial cytochrome oxidase gene expression in Alzheimer disease

Mol Brain Res

(1994)

J. Hamburger-Heyd et al.

Reduced rate of ATP catabolism and the enhanced amino acid incorporating activity of regenerating rat liver microsomes

Biochem Biophys Res Commun

(1967)

R.E. Rosensweig

Ferrohydrodynamics

(1985)

M.I. Shliomis

Sov Phys-USP

(1974)

R. Kaiser et al.

Study of ferromagnetic fluid

R. Massart

Magnetic fluids and process for obtaining them

(1982)

R. Hasegawa et al.

Magnetic iron oxide-dextran complex and process for its production

(1978)

A.H. Dutton et al.

Iron-dextran antibody conjugates: General method for for simultaneous staining of two components in high resolution immunoelectron microscopy

Proc Natl Acad Sci USA

(1979)

T. Griffin et al.

Affinity absorbents for Magnetic biospecific immunoglobulin and enzyme isolation

Appl Biochem Biotechnol

(1981)

U. Scröder

Magnetic carbohydrate particles as carriers for affinity separation purposes, eg cell separation

PCT Int Appl WO 83 03 426

(1983)

K.J. Widder et al.

Methods of magnetic separation of cells and the like, and microspheres for use therein

(1980)

J. Kandzia et al.

Cell separation: Comparison between magnetic immune microspheres (MIMS) and facs

P.F. Renshaw et al.

Ferromagnetic contrast agents: A new approach

Magn Res Med

(1986)

R.A. Whitehead et al.

Magnetic particles for use in separations

(1987)

M. Okada et al.

Immunoasay using magnetic particles

(1990)

C.H. Wang et al.

Magnetically responsive fluorescent polymer particles and application thereof

(1991)

E.V. Groman et al.

Low molecular weight carbohydrates as additives to stabilize metal oxide compositions

(1993)

A. Halbreich et al.

Nanoparticules magnétiques complées à de l'annexine et leur utilisation

A. Halbreich et al.

Magnetic maghemite nanoparticles: their preparation and application in cell sorting and characterization of cellular membranes in vitro

C. Reuteligsperger

Use of an anticoagulant as a diagnostic agent

(1991)

P. Raynal et al.

Annexin 5 as a potential regulator of annexinn 1 phosphorylation by protein kinase C. In vitro inhibition compared with quantitative data on annexin distribution in human endothelial cells

Biochem J

(1993)

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Biomedical applications of maghemite ferrofluid

Abstract

J Immunol Methods

Biochim Biophys Acta

J Biol Chem

Blood

Blood

Blood

Parasitol Today

Parasitol Today

J Psychiatr Res

Neurobiol Aging

Lancet

Drug Discovery Today

Neurobiol Aging

Neurobiol Aging

Clin Immunol Immunopathol

Psychiatry Res

Biochem Biophys Res Commun

Biochim Biophys Acta

Biochim Biophys Acta

Mol Brain Res

Biochem Biophys Res Commun

Ferrohydrodynamics

Sov Phys-USP

Study of ferromagnetic fluid

Magnetic fluids and process for obtaining them

Magnetic iron oxide-dextran complex and process for its production

Iron-dextran antibody conjugates: General method for for simultaneous staining of two components in high resolution immunoelectron microscopy

Proc Natl Acad Sci USA

Affinity absorbents for Magnetic biospecific immunoglobulin and enzyme isolation

Appl Biochem Biotechnol

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Cell separation: Comparison between magnetic immune microspheres (MIMS) and facs

Ferromagnetic contrast agents: A new approach

Magn Res Med

Magnetic particles for use in separations

Immunoasay using magnetic particles

Magnetically responsive fluorescent polymer particles and application thereof

Low molecular weight carbohydrates as additives to stabilize metal oxide compositions

Nanoparticules magnétiques complées à de l'annexine et leur utilisation

Magnetic maghemite nanoparticles: their preparation and application in cell sorting and characterization of cellular membranes in vitro

Use of an anticoagulant as a diagnostic agent

Annexin 5 as a potential regulator of annexinn 1 phosphorylation by protein kinase C. In vitro inhibition compared with quantitative data on annexin distribution in human endothelial cells

Biochem J