Efficacy of a central venous catheter (Hydrocath®) loaded with teicoplanin in preventing subcutaneous Staphylococcal infection in the mouse

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Summary

Segments (1 cm in length) of a central venous catheter (Hydrocath®) coated with teicoplanin, or uncoated, were inserted subcutaneously into mice and Staphylococcus aureus or Staphylococcus epidermidis were inoculated nearby. At 24 and 48 h after infection, the mean titre of adherent bacteria (recovered by sonication) for the teicoplanin-coated catheters was less than 10 CFU as compared to more than 104 CFU (S. aureus) and 103 CFU (S. epidermidis) from the uncoated catheters. In the S. aureus infection, teicoplanin coating also prevented the formation of abscesses which were observed around uncoated catheters.

Zusammenfassung

Ein cm lange Segmente eines zentralvenösen Katheters (Hydrocath®), die mit dem Glykopeptid Teicoplanin beschichtet waren, wurden subcutan in Mäuse implantiert. Zur Erzeugung einer Infektion wurden entweder Staphylococcus aureus oder Staphylococcus epidermidis in der Nähe der Implantationsstelle eingeimpft. 24 h bzw. 48 h nach Bakterieninokulation war die Anzahl adhärenter Bakterien (bestimmt durch Ultraschallablösung mit anschließender Keimzahlbestimmung) am Teicoplanin-beschichteten Katheter kleiner als 10 cfu, wogegen bei den unbeschichteten Kathetern Adhäsionswerte von mehr als 104 cfu (S. aureus) bzw. 103 cfu (S. epidermidis) gefunden wurden. Im Falle von S. aureus wurde bei unbeschichteten Kathetern Abszeßbildung beobachtet, bei den Teicoplaninbeschichteten Kathetern wurde keine Abszeßbildung beobachtet.

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    Marisa Berti, Lepetit Research Center, Via R. Lepetit 34, 21040 Gerenzano (VA), Italy

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