Trends in Molecular Medicine
ReviewMesenchymal stem cells: building blocks for molecular medicine in the 21st century
Section snippets
The state of the art
In the late 1980s, the technique for isolating, purifying and mitotically expanding MSCs from marrow specimens of individuals of any age was optimized 25. With an expansion potential exceeding one billion-fold in culture 26, and the subsequent availability of large numbers of MSCs, studies were initiated to further characterize these cells and their response to various bioactive molecules. Although inductive agents that cause the entrance into and progression along individual lineage pathways
Tissue protection, repair and replacement
The surgical reconstruction of any tissue will require reparative cells and the appropriate scaffold to both introduce the cells into the wound site and support tissue specific biosynthetic events. Additionally, inductive and phenotype-specific growth factors and cytokines must sequentially interact with the implanted cells and their progeny to effectively consummate a tissue engineered reformation of functional tissue. Hybrid tissues, such as articular cartilage on a bed of subchondral bone,
Acknowledgements
We thank our colleagues at Case Western Reserve University, DePuy, and elsewhere for their encouragement and scientific stimulation. Supported in part by DePuy, Inc., and grants from National Institutes of Health.
References (61)
The mesengenic process
Clin. Plastic Surg.
(1994)Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies
Bone
(1992)The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105)
Biochem. Biophys. Res. Commun.
(1999)- et al.
Mesenchymal stem cells: biology and potential clinical uses
Exp. Hematol.
(2000) Effect of donor age on osteogenic cells of rat bone marrow in vitro
Mech. Ageing Dev.
(1990)Characterization of cells with osteogenic potential from human marrow
Bone
(1992)In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells
Exp. Cell Res.
(1998)Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
Cell
(1997)Effect of rhBMP-2 on the osteogenic potential of bone marrow stromal cells from an osteogenesis imperfecta mouse (oim)
Bone
(1997)Fresh osteochondral allografts for post-traumatic knee defects: surgical technique
Oper. Tech. Orthop.
(1997)
Transcriptional mechanisms of chondrocyte differentiation
Matrix Biol.
Embryonic stem cells for medicine
Sci. Am.
Hematopoietic stem cells
Mesenchymal stem cells
J. Orthop. Res.
Multilineage potential of adult human mesenchymal stem cells
Science
CD34+ progenitor/stem cells for transplantation
Hematol. Rev.
Mesenchymal stem cell surface antigen SB-10 corresponds to activated leukocyte cell adhesion molecule and is involved in osteogenic differentiation
J. Bone Miner. Res.
Age-related changes in osteogenic stem cell in mice
J. Bone Miner. Res.
The effect of aging on bone formation in porous hydroxyapatite: biochemical and histological analysis
J. Bone Miner. Res.
Age-related bone loss: a hypothesis and initial assessment in mice
Clin. Orthop. Rel. Res.
Bone progenitor cell deficits and the age-associated decline in bone repair capacity
Calcif. Tissue Int.
Bone deficit in ovariectomized rats
J. Clin. Invest.
Stem cell plasticity in mammals and transdetermination in Drosophila: common themes?
Stem Cells
Purified hematopoietic stem cells can differentiate into hepatocytes in vivo
Nat. Med.
Hematopoietic potential of stem cells isolated from murine skeletal muscle
Proc. Natl. Acad. Sci. U. S. A.
Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats – similarities to astrocyte grafts
Proc. Natl. Acad. Sci. U. S. A.
Chondrocyte-to-osteocyte transformation in grafts of perichondrium-free epiphyseal cartilage
Clin. Orthop. Rel. Res.
Adipocytic cells cultured from marrow have osteogenic potential
J. Cell Sci.
Translating stem and progenitor cell biology to the clinic: barriers and opportunities
Science
Multipotential cells in the bone marrow stroma: regulation in the context of organ physiology
Crit. Rev. Eukaryot. Gene Expr.
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