Notes & TipsPumpless steady-flow microfluidic chip for cell culture
Section snippets
Acknowledgments
This research was supported by the Korea–New Zealand Joint Research Program funded by the Ministry of Education, Science, and Technology (2011K000700) and was further supported by the Industrial Strategic Technology Development Program (10035197) funded by the Ministry of Knowledge Economy of Korea.
References (19)
- et al.
Monolithic fabrication of three-dimensional microfluidic networks for constructing cell culture array with an integrated combinatorial mixer
Sens. Actuat. B
(2008) - et al.
Microfluidic system for automated cell-based assays
J. Assoc. Lab. Autom.
(2007) - et al.
The use of flow perfusion culture and subcutaneous implantation with fibroblast-seeded PLLA–collagen 3D scaffolds for abdominal wall repair
Biomaterials
(2010) - et al.
Effect of fiber diameter, pore size, and seeding method on growth of human dermal fibroblasts in electrospun poly(3-caprolactone) fibrous mats
Biomaterials
(2010) - et al.
Capillary blood cell velocity in human skin capillaries located perpendicularly to the skin surface: measured by a new laser Doppler anemometer
Microvasc. Res.
(1996) - et al.
A practical guide to microfluidic perfusion culture of adherent mammalian cells
Lab Chip
(2007) - et al.
Microfluidic cell culture systems for drug research
Lab Chip
(2010) - et al.
Fundamentals of microfluidic cell culture in controlled microenvironments
Chem. Soc. Rev.
(2010) - et al.
Differentiation-on-a-chip: a microfluidic platform for long-term cell culture studies
Lab Chip
(2005)
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