Elsevier

Biomaterials

Volume 32, Issue 11, April 2011, Pages 2878-2884
Biomaterials

The influence of the scaffold design on the distribution of adhering cells after perfusion cell seeding

https://doi.org/10.1016/j.biomaterials.2011.01.023Get rights and content
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Abstract

In natural tissues, the extracellular matrix composition, cell density and physiological properties are often non-homogeneous. Here we describe a model system, in which the distribution of cells throughout tissue engineering scaffolds after perfusion seeding can be influenced by the pore architecture of the scaffold. Two scaffold types, both with gyroid pore architectures, were designed and built by stereolithography: one with isotropic pore size (412 ± 13 μm) and porosity (62 ± 1%), and another with a gradient in pore size (250–500 μm) and porosity (35%–85%). Computational fluid flow modelling showed a uniform distribution of flow velocities and wall shear rates (15–24 s-1) for the isotropic architecture, and a gradient in the distribution of flow velocities and wall shear rates (12–38 s-1) for the other architecture. The distribution of cells throughout perfusion-seeded scaffolds was visualised by confocal microscopy. The highest densities of cells correlated with regions of the scaffolds where the pores were larger, and the fluid velocities and wall shear rates were the highest. Under the applied perfusion conditions, cell deposition is mainly determined by local wall shear stress, which, in turn, is strongly influenced by the architecture of the pore network of the scaffold.

Keywords

Scaffolds
Microstructure
Cell adhesion
Confocal microscopy
Image analysis
Computational fluid dynamics

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