Chitosan nanoparticles crosslinked by glycidoxypropyltrimethoxysilane for pH triggered release of protein

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Abstract

pH-responsive-chitosan nanoparticles for the control release of protein drug were prepared by combining two-step crosslinking method, in which chitosan was subsequently crosslinked by sodium tripolyphosphate (TPP) and glycidoxypropyltrimethoxysilane (GPTMS). Compared with TPP crosslinked chitosan particles, the two-step crosslinked nanoparticles were not only pH-responsive but also more stable in wide pH range. Fluorescein isothiocyanate (FITC) labeled anti-human-IgG antibody was used as a model protein drug for evaluating the control release profile of the nano-carrier. The amount of released antibody increased from 5.6% to 50% when the pH of solution shifted from 7.4 to 6.0. The results suggest the possible application of the nanoparticles as pH-responsive drug delivery materials.

Section snippets

Experimental

To obtain the low molecular weight chitosan (LMWC), 5 g chitosan (Mr = 8 × 105, degree of deacetylation 96%, purchased from Yuhuan Ocean Biochemical Corp., Zhejiang) was dissolved in 150 mL 3% (v/v) acetic acid, then 50 mL 6% hydrogen peroxide solution was added dropwise into the chitosan solution. The mixture was stirred and heated in a water bath of 40 °C. After 12 h, the partially degraded chitosan was precipitated by adding 10 mol/L of NaOH until the pH reach 10.0, followed by washing with deionized

Result and discussion

In the nanoparticles preparing procedure, TPP was used in first step as the ionic crosslinker. It has been reported that the molecular weigh of chitosan nanoparticles could be significantly affected by the molecular weight of chitosan. In this work, Chitosan with molecular weight of 50 KDa could be obtained after 12 h degradation. By adding 0.1% TPP into the solution of 50 KDa LMWC, nano-sized particles with mean diameter of 39.8 nm could be formed. The size of the beads increased to average of 43.9

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