J Appl Biomed 13:239-248, 2015 | DOI: 10.1016/j.jab.2015.03.004

Self-assembly of carrageenin-CaCO₃ hybrid microparticles on bacterial cellulose films for doxorubicin sustained delivery

Maximiliano L. Cacicedo^a, Karina Cesca^b, Valeria E. Bosio^a, Luismar M. Porto^b, Guillermo R. Castro^a,*

^a Nanobiomaterials Laboratory, Institute of Applied Biotechnology (CINDEFI, CONICET CCT La Plata), Department of Chemistry, School of Sciences, Universidad Nacional de La Plata, Calle 47 y 115, CP 1900, Ciudad de La Plata, Argentina

^b Integrated Technologies Laboratory (InteLAB), CTC/EQA, Universidad Federal de Santa Catarina, Florianopolis, Brazil

Stereospecific nucleation of mesoporous hybrid microspheres composed of CaCO₃ and carrageenan was appended to one side of bacterial cellulose membrane synthesized in static cultures of Gluconacetobacter hansenii to develop an implantable drug delivery device. The synthesis of the hybrid microparticles proceeds by self-assembly mechanism in the presence of calcium and contains tailorable amounts of doxorubicin. However, in the absence of the particles, doxorubicin was distributed along the BC film, but without control release of drug. Infrared spectroscopy, confocal and scanning electron microscopies analyses demonstrate that the doxorubicin is entrapped inside the hybrid particles with approximately 80% drug loading compared to the 11% obtained for native bacterial cellulose. Doxorubicin content in the hybrid particles can be increased by a factor of 10 (from 258.6 to 2586.3 nmol ml^-1), and also by the quantities of particles regulated by the CaCO₃-carrageenan content and the physicochemical microenvironment. The hybrid BC system can be considered as smart device since the kinetic release of doxorubicin from the hybrid cellulose system rise from 1.50 to 2.75 μg/membrane/day when the pH decreases from 7.4 to 5.8 at 37 °C, a pathologic simulated environment. The hybrid microparticle system can be potentially used as an implantable drug delivery system for personalized oncological therapies.

Keywords: Self-assembly; Nanocomposites; Hybrid microparticles; Bacterial cellulose; Doxorubicin; Smart chemotherapy

Received: November 13, 2014; Revised: March 6, 2015; Accepted: March 9, 2015; Published: July 1, 2015  Show citation