Basic ResearchDeciduous and Permanent Dental Pulp Mesenchymal Cells Acquire Hepatic Morphologic and Functional Features In Vitro
Section snippets
Cell Culture
All protocols for this study were reviewed and approved by the Research Ethics Committee of Nippon Dental University. Deciduous (n = 5) and upper wisdom teeth (n = 5) were supplied by patients visiting Nippon Dental University Hospital and Iino Dental Office, Tokyo, Japan. Informed consent was obtained from the subjects who agreed to participate voluntarily in this study. Deciduous teeth were collected after extraction. The pulp was accessed through the resorbed tooth root canal and extracted
Stem Cell Markers in Dental Pulp
The mesenchymal cell cultures retained their fibroblast-like, spindle-shaped morphology when they were grown in normal culture conditions without growth factors. Immunocytochemical studies were performed to characterize clonogenic populations of wisdom tooth pulp cells (WTPCs) and deciduous tooth pulp cells (DTPCs) using a panel of antibodies specific to antigens associated with different phenotypes. Mesenchymal cell cultures were positive for the following pluripotent cell markers: CD117, a
Discussion
During development, interactions between ectodermal cells of the enamel organ and ectomesenchymal (neural crest-derived) cells of the dental papilla lead to the differentiation of ameloblasts and odontoblasts (16). It has been shown that progenitor cells exist in the mature tooth and that they are recruited from dental pulp to differentiate into connective tissue cells and terminally differentiated odontoblasts 1, 2. Miura et al (5) have shown that DPSCs are capable of differentiating into
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