Basic ResearchHistologic Analysis of the Influence of a Gelatin-based Scaffold in the Repair of Immature Dog Teeth Subjected to Regenerative Endodontic Treatment
Section snippets
Methods
The research project was approved by the Animal Ethics Screening Committee of the Federal University of Santa Catarina (Florianópolis, Santa Catarina, Brazil). The second, third, and fourth mandibular premolars and the second and third maxillary premolars (n = 60 roots) of 3 beagle dogs aged 5–6 months were used.
Animals received preanesthesia with 0.5 mg/kg acepromazine hydrochloride (Ouro Fino, Cravinhos, SP, Brazil) and 4 mg/kg pethidine (Cristália, São Paulo, SP, Brazil) via intramuscular
Results
Intra- and interexaminer agreement yielded kappa = 1 for all parameters except inflammatory reaction, for which scores were 0.89 and 0.87, respectively.
At the end of the experiment, 3 teeth were lost in the blood clot group: 1 because of severe destruction of supporting tissues; 1 during histologic processing; and 1 because the MTA plug moved apically, preventing the assessment of the parameters under investigation. One tooth from the blood clot + Gelfoam group was also lost because of a
Discussion
Some previous studies have shown the usefulness and advantages associated with the use of scaffolds in regenerative endodontic procedures 24, 26. Scaffolds provide a structure and an initial support onto which cells can adhere, proliferate, differentiate, and form an extracellular matrix (27).
In the quest for ideal scaffolds, several materials have been tested 9, 25, 26, 28. However, most of them are difficult to acquire and have a high cost (24). The need to find a material that meets all the
Acknowledgments
The authors deny any conflicts of interest related to this study.
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Cited by (23)
Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
2021, Polymer TestingCitation Excerpt :Gelatin scaffolds were found to promote the adhesion and growth of human dental pulp cells (hDPCs). However, their high degradation rate and absence of odontogenic induction limited their application in pulp regeneration [12,13]. Our previous studies showed that bioactive glass (BG) not only had good mineralization and osteogenesis properties but also excellent odontogenesis and cell chemotaxis performances [14–17].
Histologic Findings of a Human Immature Revascularized/Regenerated Tooth with Symptomatic Irreversible Pulpitis
2017, Journal of EndodonticsScaffolds for Dentin–Pulp Complex Regeneration
2024, Medicina (Lithuania)Efficacy of propolis and triple antibiotic paste as intra-canal medicaments for revascularisation of immature teeth in dogs: a comparative study
2023, European Archives of Paediatric Dentistry