Elsevier

Materials Science and Engineering: C

Volume 35, 1 February 2014, Pages 106-114
Materials Science and Engineering: C

Preparation and characterisation of nanophase Sr, Mg, and Zn substituted hydroxyapatite by aqueous precipitation

https://doi.org/10.1016/j.msec.2013.10.015Get rights and content

Highlights

  • Sr, Mg as well as Zn doped HA was precipitated under ‘identical’ conditions.

  • Mg2 + ions were observed to inhibit HA nucleation and crystallisation.

  • Apatite growth on substrates in SBF was confirmed to be a positive result of bioactivity.

  • Interferometry results did not direct correlate to increased apatite growth in SBF.

  • Live/dead assay confirmed the viability of MC3T3 cells on all HA substrates.

Abstract

Hydroxyapatite (HA) substituted with 2 mol% Sr, 10 mol% Mg, and 2 mol% Zn were precipitated under identical alkaline conditions (pH 11) at 20°C from an aqueous solution. As-synthesised materials were confirmed to be phase pure by XRD and samples prepared in air contained surface adsorbed CO2 as observed by FTIR. SEM studies revealed a globular morphology and agglomeration behaviour, typical of precipitated nHA. EDS spectra confirmed nominal compositions and substitution of Sr, Mg and Zn. At the levels investigated cationic doping was not found to radically influence particle morphology. An indication of the potential in-vivo bioactivity of samples was achieved by analysing samples immersed in SBF for up to 28 days by interferometry and complementary SEM micrographs. Furthermore, a live/dead assay was used and confirmed the viability of seeded MC3T3 osteoblast precursor cells on HA and substituted HA substrates up to 7 days of culture.

Keywords

Bioceramics
Substituted hydroxyapatite
Aqueous precipitation
Simulated body fluid
Osteoblasts

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