In vitro cytotoxicity effect and antibacterial performance of human lung epithelial cells A549 activity of Zinc oxide doped TiO₂ nanocrystals: Investigation of bio-medical application by chemical method

Highlights

• Oxidative stress and DNA damage to human lung epithelial cells (A549) lines

• Modifications of the cell membrane of pulmonary A549 cell lines nanoparticles

• All peaks due to the enhancement of radiative recombination in luminescent process

• ZnTi bending vibrations are identified at 850, 780 and 610 cm^− 1.

• Antibacterial activity over a broad range of bacterial species against in Stre. Mut

Abstract

We report the synthesis of high quality ZnO doped TiO₂ nanocrystals by chemical method at room temperature (RT), it can cause serious oxidative stress and DNA damage to human lung epithelial cells (A549) lines. Our aim in this study, to reduce the cytotoxicity effect of ZnO doped TiO₂ nanocrystals are widely in biological fields. Several studies have been performed to understand the influence of ZnO doped titanium dioxide (TiO₂-NPs) on cell function; however the effects of nanoparticle against to exposure on the cell membrane have been duly addressed fascinatingly so far. However, In this interaction, which may alter cell metabolism and integrity, it is one of the importance to understand the modifications of the cell membrane, mechanisms of pulmonary A549 cell lines nanoparticles were uptake and the molecular pathway during the initial cell responses are still unclear and much more investigative efforts are need to properly characterize the ZnO doped titanium dioxide nanoparticles were reported successfully. In particular of the epithelial cells, upon particles are exposed human pulmonary epithelial cells (A549) to various concentrations of composition, structure and morphology of the nanocrystals were analyzed by X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM). XRD assessed the crystal structure of the nanocrystals which identified peaks associated with (002), (100) and (101) planes of hexagonal wurtzite-type ZnO with lattice constants of a = b = 3.249 Å and c = 5.219 Å. The IR results showed high purity of products and indicated that the nanocrystals are made up of TiO and ZnO bonds. The Photoluminescence (PL) spectra are dominated by a strong narrow band edge emission tunable in the blue region of the visible spectra indicating a narrow size distribution of ZnO/TiO₂ nanocrystals which exhibits antibacterial activity over a broad range of bacterial species and in particular against Stre. Mut where it out competes four other commonly used E.coli, Pse. Aug, Stre. Mut and Salm. sps, well as testing four different appropriate concentration from the results showed a significant gain in viable cell numbers of all four bacteria species, with 5 mM and 10 mM being the most effective and 2 mM being the worst, where it provided an effective improvement from the growth mechanism has been also proposed to the current interest of these nanocrystals will discussed in detail.