Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole

doi:10.1016/j.nano.2009.06.005

Nanomedicine: Nanotechnology, Biology and Medicine

Volume 5, Issue 4, December 2009, Pages 382-386

https://doi.org/10.1016/j.nano.2009.06.005 Get rights and content

Abstract

Silver nanoparticles (Ag-NPs) are known to have inhibitory and bactericidal effects. Resistance of fungal infections has emerged in recent years and is a major health problem. Here, we report the extracellular biosynthesis of Ag-NPs using a common fungus, Alternaria alternata. Also in this study, these nanoparticles were evaluated for their part in increasing the antifungal activity of fluconazole against Phoma glomerata, Phoma herbarum, Fusarium semitectum, Trichoderma sp., and Candida albicans. The antifungal activity of fluconazole was enhanced against the test fungi in the presence of Ag-NPs. Fluconazole in combination with Ag-NPs showed the maximum inhibition against C. albicans, which was confirmed from the increase in fold area of inhibition, followed by P. glomerata and Trichoderma sp., which showed less increase in the fold area, whereas no significant enhancement of activity was found against P. herbarum and F. semitectum.

From the Clinical Editor

The antifungal activity of fluconazole was enhanced in presence of silver nanoparticles against the test fungi. Fluconazole in combination with Ag-NPs showed the maximum inhibition against C. albicans, followed by P. glomerata and Trichoderma sp. No significant enhancement of activity was found against P. herbarum and F. semitectum.

Section snippets

Fungi

Alternaria alternata (Fr.) Keissler (MTCC-6572; used for the synthesis of Ag-NPs), Phoma glomerata (MTCC-2710), Phoma herbarum (MTCC-2319), Fusarium semitectum (USM-3794), Trichoderma sp. (MTCC-3201), and Candida albicans (NCIM-3100; used for evaluation of Ag-NP sensitivity) were procured from the Microbial Type Culture Collection (MTCC) center, IMTECH, Chandigarh, India; National Chemical Laboratory (NCL), Pune, India; and the Culture Collection Center of University of Sains, Malaysia. These

Results and discussion

The fungal cell filtrate after addition of aqueous AgNO₃ (1 mM) was subjected to optical measurements by UV-Vis spectrophotometer; this analysis showed an absorbance peak at 420 nm (Figure 1), which was specific for the silver nanoparticles.

The exact mechanism of the synthesis of Ag-NPs was not known, but later it was hypothesized that the silver ions required the NADH-dependent nitrate reductase enzyme for their reduction,29, 30 which was secreted by the fungus in its extracellular

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CommunicationFungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole

Abstract

From the Clinical Editor

Section snippets

Fungi

Results and discussion

Chem Biol

Adv Drug Deliv Rev

Electrochim Acta

Biomaterials

Biotechnol Adv

Nanomedicine: NBM

Nanomedicine: NBM

Mater Sci Eng B

Trends Biotechnol

Trends Biotechnol

Solid State Commun

Bioresource Technol

Colloid Surf B

Physics B

Antimicrobial effects of metal ions (Ag+, Cu2+, Zn2+) in hydroxyapatite

J Mater Sci Mater Med

Inactivation of enveloped viruses by a silver-thiosulfate complex

Metal-Based Drugs

Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria

Curr Nanosci

Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli

Appl Environ Microbiol

Nanosilver as substance biostabilising footwear materials in the foot mycosis prophylaxis

Mikologia Lekarska

Antifungal effect of silver nanoparticles on dermatophytes

J Microbiol Biotechnol

Antifungal activity and mode of action of silver nano-particles on Candida albicans

Biometals

Communication
Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole