ReviewStructural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes
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Keywords
Glycoside hydrolase
Endoglucanase
GH family 12
Protein engineering
Protein structure
Thermal stability
Abbreviations
CBH
Cellobiohydrolase
CBM
Cellulose binding module
CD
circular dichroism
cd
catalytic domain
DP
Degree of polymerization
EG
Endoglucanase
F. javanicum
Fusarium javanicum
GH
Glycoside hydrolase
GH 12
Glycoside hydrolase family 12
G. roseum
Gliocladium roseum
G2
Cellobiose
G2SG2
Methyl 4-S-β-cellobiosyl-4-thio-β-cellobioside
G4
Cellotetraose
G5
Cellopentaose
H. grisea
Humicola grisea
H. jecorina
Hypocrea jecorina
H. koningii
Hypocrea koningii
H. schweinitzii
Hypocrea schweinitzii
NAG
N-acetyl-glucosamine
NCS
Non-crystallographic symmetry
oNPC
o-Nitrophenyl-β-cellobioside
pNPC
p-Nitrophenyl-β-cellobioside
S. sp. 11AG8
Streptomyces sp. 11AG8
Tm
The mid-point of thermal denaturation
T. reesei
Trichoderma reesei
WT
Wild type
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