Characterization and functional validation of glyoxalase II from rice
Section snippets
Cloning and sequence analysis of OsglyII
The rice glyII cDNA (OsglyII) was cloned by PCR based approach from rice cDNA library [30]. Sequence analysis revealed one full length cDNA clone of OsglyII (Accession No. AY054407) based on homology with Accession No. U90927 [23]. For homology analysis, BLAST searches were conducted using GenBank. GlyII protein sequences were aligned using the ClustalW multiple alignment program (MacVector). Dendrogram was prepared from amino acid sequences between various glyII sequences already reported in
Molecular cloning and sequence comparison of OsglyII cDNA with other glyII in the data bank
The sequence analysis of the cloned gene encoding for glyII enzyme of glyoxalase pathway from rice (OsglyII) revealed that the cDNA clone is 1623 bp long with an open reading frame of 1010 bp (representing the full length glyII coding sequence) and a 108 bp long 5′ UTR and 504 bp long 3′ UTR. The deduced amino acid sequence length for glyII was found to be 336 residues. The estimated molecular mass of the protein was ∼37 kDa with an isoelectric point of 8.08. The glyII from Arabidopsis and spinach
Acknowledgments
We thank Professor Ray Wu, Cornell University, USA for valuable suggestions and critical reading of the manuscript. Thanks are also due to Drs. F. White and B.W. Porter, Kansas State University, USA, for the initial glyoxalase II clone. The financial support by the Department of Biotechnology (DBT, New Delhi) Rice Network Project, International Foundation for Science, Sweden research grant to SLS-P, DBT Post-Doc fellowship to S.K.Y. and grants from the International Centre for Genetic
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Glyoxalase II
2023, Metalloenzymes: From Bench to BedsideOsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance
2022, Plant Physiology and BiochemistryCitation Excerpt :Therefore, the glyoxalase are life essential proteins that physiologically modulate the oxidative damage caused by methylglyoxal (MG) (Kargatov et al., 2018; Rai et al., 2021; Thornalley, 2003). Glyoxalase pathway enzymes are widely studied in plant systems (Singla-Pareek et al., 2003; Yadav et al., 2007). For instance, Brassica GlyI heterologous expression in tobacco revealed the potential of these genes in conferring enhanced tolerance to MG and high salt conditions (Veena et al., 1999).
Molecular and biochemical characterization of All0580 as a methylglyoxal detoxifying glyoxalase II of Anabaena sp. PCC7120 that confers abiotic stress tolerance in E. coli
2019, International Journal of Biological MacromoleculesMethylglyoxal – a signaling molecule in plant abiotic stress responses
2018, Free Radical Biology and MedicineGlobal proteomic analysis of advanced glycation end products in the Arabidopsis proteome provides evidence for age-related glycation hot spots
2017, Journal of Biological ChemistryCitation Excerpt :As Asc and GSH are involved in such protective systems (41), the increase of total ascorbate levels (Fig. 2F) can be explained by its higher consumption for the reduction of overproduced hydroperoxides. The moderate age-dependent decrease of GSH/GSSG ratio and total glutathione contents (Fig. 2, E and G) might indicate depletion of GSH due to scavenging of OH•, decrease of GRcyt leaf transcript levels (Fig. 3A), and involvement of GSH in detoxication of α-dicarbonyls by the glyoxalase system (42). The latter assumption is confirmed by the higher expression levels and activity of GLX2 (Fig. 3, B and C) without any effect on GO and MGO leaf contents (Fig. 4B).
Involvement of glyoxalases and glutathione reductase in conferring abiotic stress tolerance to Jatropha curcas L.
2017, Environmental and Experimental BotanyCitation Excerpt :In the present study, there was an increased viability in cells exposed to MG and abiotic stress, due to the presence of JcGLYI, II and JcGR which could act as stress protective enzymes in bacteria and yeast by overcoming the harmful effects of abiotic stress. Abiotic stress tolerance is a multigenic trait which is controlled at multiple loci and glyoxalases are considered to be the primary components involved in plant defence mechanism where they are expressed in a time-dependent and differential manner during various abiotic stresses such as salinity, heat, desiccation, cold, metal and abscisic acid/salicylic acid treatment (Kaur et al., 2014; Yadav et al., 2007). The upregulation of glyoxalases and other antioxidant systems such as GR was observed when plants were exogenously treated with selenium, nitric oxide, proline and glycine betaine by alleviating drought and salinity stress in rape seed, wheat and cultured tobacco cells, respectively (Hasanuzzaman and Fujita 2011; Hoque et al., 2008; Mostofa and Fujita 2013).
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Present address: Biotechnology Division, Institute of Himalayan Bioresource Technology, Palampur 176061, India.
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Both the authors have contributed equally to this work.