Structure and properties of chitosan derivatives modified calcium polyphosphate scaffolds

doi:10.1016/j.polymdegradstab.2010.04.004

Polymer Degradation and Stability

Volume 95, Issue 7, July 2010, Pages 1205-1210

https://doi.org/10.1016/j.polymdegradstab.2010.04.004 Get rights and content

Abstract

Organic and inorganic composite material is becoming a solution on making the mechanical and degradation properties of biomaterial more suited. Porous calcium polyphosphate was immersed into different concentrations of carboxymethyl chitosan before immersing 10% alginate dialdegyde. After freeze-drying, the scaffolds were performed in physiologic saline. At stated day, the weightloss, Ca²⁺ concentration, pH value and morphology were measured. The biocompatibility of the composite was demonstrated by extract and direct contact tests. As the results showed, the degradation rates of composites were faster, and the compressive strength became bigger because of the cross-linked network formed by Carboxymethyl chitosan (CMC) and alginate dialdehyde (ADA). The pH value of composite was higher than that of calcium polyphosphate (CPP) due to the organic part of composite’s pH was in slight alkaline. From the SEM, the cross-linked network structure could be observed clearly. Because the glycosaminoglycans-like chains in CMC molecules, which are typically presented in extracellular matrix (ECM), extractions of composite material gave the cells good adhesion and growth condition. All the results testified the composite scaffold was a good candidate for bone repair.

Section snippets

Introductions

Bone defect repair or reconstruction is becoming a major issue in orthopedic surgery. Many inorganic scaffolds have been studied in order to repair or substitute the injured parts because of the similarities of their components with bone in recent years. However, unmatched ratio between the mechanical and degradation properties limited these materials’ applications. How to make the mechanical and degradation properties more suitable is a focus in bone tissue engineering. Composing inorganic and

Materials

CS (with a MW of about 1.0 × 105 and a degree of deacetylation, Zhejiang Aoxing, China). Sodium alginate (low viscosity grade, 495 cps at 25 °C, Zhejiang Jinyan, China). Carboxymethyl chitosan (with a MW of about 3.0 × 10⁵, Zhejiang Aoxing, China). Diphenyl tetrazolium bromide (MTT) was obtained from Sigma–Aldrich (St. Louis, MO, USA). All other chemicals of the analytical reagent were used and obtained from Kelong Co. (Chengdu, China).

Synthesis of porous CPP scaffold

The process of producing CPP powders was followed as Kai

Results and discussion

There were many –OH on the surface of CPP, which could be bonded with the –COOH or other groups in CMC chains in aqueous solution and to form good packages. On the other hand, chitosan could only be dissolved in acidic fluids where the H⁺ was more active than chitosan molecules to combine with the –OH on the surface of CPP. So that was the reason we used CMC as the transition between CPP and chitosan. The carboxyl in CMC could react with the amido in chitosan.

Conclusion

Our work reported a simple fabrication of multi-layer composed CPP composite scaffolds. The results demonstrated that the packages of CMC and chitosan could improve the degradation, mechanical properties of CPP. In vitro MG-63 cultured with the extractions of scaffolds revealed the composite materials better cell proliferation. Further studies will pay attention to the cell direct contact and molecular level expression. CPP/CMC or CPP/CMC/CS composite materials is a promising way to be used in

Acknowledgement

This work was supported by the National Science Foundation of China (No.30870614 and No. 30870616) and National Science Foundation of Jiangsu Province, China (BK2008152). SEMs were provided by the Center of Forecasting and Analysis of Sichuan University.

References (26)

W.W. Thein-Han et al.
Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering
Acta Biomaterialia
(2009)
K. Qiu et al.
Effect of strontium ions on the growth of ROS17/2.8 cells on porous calicum polyphosphate scaffolds
Biomaterials
(2006)
R.A. Kandel et al.
Repair of osteochondral defects with biphasic cartilage–calcium polyphosphate constructs in a sheep model
Biomaterials
(2006)
M.D. Grynpas et al.
Porous calcium polyphosphate scaffolds for bone substitute applications in vivo studies
Biomaterials
(2002)
C. Chatelet et al.
Influence of the degree of acetylation on some biological properties of chitosan films
Biomaterials
(2001)
E. Khor et al.
Implantable application of chitin and chitosan
Biomaterials
(2003)
D. Wendt et al.
Bioreactor-based engineering of osteochondral grafts: from model systems to tissue manufacturing
J Biosci and Bioeng
(2005)
R.A.A. Muzzarelli
Carboxymethylated chitins and chitosans
Carbohydr Polymers
(1988)
M.S. Park et al.
Mechanical properties of bioactive amorphous calcium phosphate/methacrylate composites
Dent Mater
(1998)
Hockin H.K. Xu et al.
Fast setting calcium phosphate–chitosan scaffold: mechanical properties and biocompatibility
Biomaterials
(2005)

Jennifer L. Moreau et al.

Mesenchymal stem cell proliferation and differentiation on an injectable calcium phosphate–Chitosan composite scaffold

Biomaterials

(2009)

G.Y. Lu et al.

Degradation of covalently cross-linked carboxymethyl chitosan and its potential application for peripheral nerve regeneration

Eur Polym J

(2007)

S. Oprea et al.

Evaluation of physico-mechanical properties of precipitated polyurethane films in medium of free radical agents

Eur Polym J

(2002)

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Structure and properties of chitosan derivatives modified calcium polyphosphate scaffolds

Abstract

Section snippets

Introductions

Materials

Synthesis of porous CPP scaffold

Results and discussion

Conclusion

Acknowledgement

Acta Biomaterialia

Biomaterials

Biomaterials

Biomaterials

Biomaterials

Biomaterials

J Biosci and Bioeng

Carbohydr Polymers

Dent Mater

Biomaterials

Biomaterials

Eur Polym J

Eur Polym J