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Identification of the cry gene in Bacillus thuringiensis strain WZ-9 and its toxicity against Henosepilachna vigintioctomaculata

Published online by Cambridge University Press:  03 March 2009

Song Ping ,
Wang Qin-Ying ,
Wu Hui-Xian ,
Lu Xiu-Jun  and
Wang Yong
Song Ping
Affiliation:
College of Plant Protection, Biocontrol Center of Plant Diseases and Plant Pests of Hebei Province, Agricultural University of Hebei, Baoding 071001, China
Wang Qin-Ying*
Affiliation:
College of Plant Protection, Biocontrol Center of Plant Diseases and Plant Pests of Hebei Province, Agricultural University of Hebei, Baoding 071001, China
Wu Hui-Xian
Affiliation:
College of Plant Protection, Biocontrol Center of Plant Diseases and Plant Pests of Hebei Province, Agricultural University of Hebei, Baoding 071001, China
Lu Xiu-Jun
Affiliation:
College of Plant Protection, Biocontrol Center of Plant Diseases and Plant Pests of Hebei Province, Agricultural University of Hebei, Baoding 071001, China
Wang Yong
Affiliation:
Central Laboratory of Tianjin Academy of Agricultural Sciences, Tianjin 300192, China
*
*Corresponding author. E-mail: wqinying@hebau.edu.cn
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Abstract

Bacillus thuringiensis strain WZ-9, isolated from soil in Hebei province, China, was effective against Henosepilachna vigintioctomaculata larvae. The strain presented bipyramidal crystals with a protein band of 130 kDa in SDS–PAGE. The pH changes of the culture media showed important fluctuations during the 24 h growth cycle. The pH varied less in log and stationary phases than it did in the exponential phase. Bioassay results showed that the WZ-9 strain was only harmful to larvae of H. vigintioctomaculata and not to either adults of H. vigintioctomaculata or other several lepidopteran and coleopteran insects. LC50 to second-instar larvae of H. vigintioctomaculata was 2.95×107 cells/ml after 72 h. Genotypic investigations showed that this strain possessed the cry7 gene. Sequence analysis demonstrated that the encoding gene contained an open reading frame (ORF) of 3414 bp and encoded 1138 amino acid residues. The deduced amino acid sequence was 99.65% identical to that of the reported Cry7Ab2 sequences. This gene was designated by the Bt δ-endotoxin nomenclature committee as Cry7Ab3 with accession number BI 1015188 in the GenBank database.

Keywords

Bacillus thuringiensisCry7AbHenosepilachna vigintioctomaculata
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 245 - 250
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(3): 515–520

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