Abstract
The repair of ultraviolet-B radiation induced damage to the structure and function of Photosystem II was studied in the cyanobacterium Synechocystis sp. PCC 6803. UV-B irradiation of intact Synechocystis cells results in the loss of steady-state oxygen evolution, an effect accompanied by a parallel loss of both D1 and D2 protein subunits of the Photosystem II reaction centre. Transfer of the UV-irradiated cells to normal growth conditions under visible light results in partial recovery of the inhibited oxygen evolving activity and restoration of the lost D1 and D2 proteins. The extent of recovery decreases with increasing degree of damage: after 50% inhibition, the original activity is completely restored within 2 hours. In contrast, after 90–95% inhibition less than half of the original activity is regained during a 4 hour recovery period. The translation inhibitor lincomycin completely blocks the recovery process if added after the UV-B treatment, and accelerates the kinetics of activity loss if added before the onset of UV-B irradiation. Substantial retardation of recovery and acceleration of activity loss is also observed if the very low intensity short wavelength contribution (λ<290 nm) is not filtered out from the UV-B light source. It is concluded that in intact cells UV-B induced damage of the Photosystem II complex can be repaired. This process is the first example of simultaneous D1 and D2 protein repair in Photosystem II, and considered to function as an important defence mechanism against detrimental UV-B effects in oxygenic photosynthetic organisms. De novo synthesis of the D1 and D2 reaction centre subunits is a key step of the repair process, which itself can also be inhibited by ultraviolet light, especially by the short wavelength UV-C components, or by high doses of UV-B.
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References
Adir N, Shochat S and Ohad I (1990) Light-dependent D1 protein synthesis and translocation is regulated by reaction center II. J Biol Chem 265: 12563-12568
Aro E-M, Virgin I and Andersson B (1993) Photoinhibition of Photosystem II. Inactivation, protein damage and turnover. Biochim Biophys Acta 1143: 113-134
Barbato R, Frizzo A, Friso G, Rigoni F and Giacometti GM (1992) Photoinduced degradation of the D1 protein in isolated thylakoids and various Photosystem II particles after donor-side inactivations. FEBS Lett 304: 136-140
Barbato R, Shipton CA, Giacometti GM and Barber J (1991) New evidence suggests that the initial photoinduced cleavage of the D1-protein may not occur near the PEST sequence. FEBS Lett 290: 162-166, 1991.
Bornman JF (1989) Target sites of UV-B radiation in photosynthesis of higher plants. J Photochem Photobiol B4: 145-158
De Las Rivas J, Andersson B and Barber J (1992) Two sites of primary degradation of the D1 protein induced by acceptor or donor side photoinhibition in photosystem two core complexes. FEBS Lett 301: 246-252
Friso G, Spetea C, Giacometti GM, Vass I and Barbato R (1993) Degradation of Photosystem II reaction center D1-protein induced by UVB irradiation in isolated thylakoids. Identification and characterization of C-and N-terminal breakdown products. Biochim Biophys Acta 1184: 78-84
Giacometti GM, Barbato R, Chiaramonte S, Friso G and Rigoni F (1996) Effects of ultraviolet-B radiation on Photosystem II of the cyanobacterium Synechocystissp. PCC 6803. Eur J Biochem 242: 799-806
Greenberg BM, Gaba V, Canaani O, Malkin S, Mattoo AK and Edelman M (1989) Separate photosensitizers mediate degradation of the 32-kDa Photosystem II reaction centre protein in visible and UV spectral regions. Proc Natl Acad Sci USA 86: 6617-6620
Greenberg BM, Gaba V, Mattoo AK and Edelman M (1987) Identification of a primary in vivo degradation product of the rapidlyturning-over 32 kd protein of Photosystem II. EMBO J 6: 2865-2869
Harm, W (1980) Biological Effects of Ultraviolet Radiation. Cambridge University Press, London
Hideg É, Sass L, Barbato R and Vass I (1993) Inactivation of oxygen evolution by UV-B irradiation. A thermoluminescence study. Photosynth Res 38: 455-462
Jansen MAK, Gaba V, Greenberg BM, Mattoo AK and Edelman M (1996) Low threshold levels of ultraviolet-B in a backgound of photosynthetically active radiation trigger rapid degradation of the D2 protein of Photosystem-II. Plant J 9(5): 693-699
Kyle DJ, Ohad I and Arntzen CJ (1984) Membrane protein damage and repair: Selective loss of a quinone-protein function in chloroplast membranes. Proc Natl Acad Sci USA 81: 4070-4074
Melis A, Nemson JA and Harrison MA (1992) Damage to functional components and partial degradation of Photosystem II reaction center proteins upon chloroplast exposure to ultraviolet-B radiation. Biochim Biophys Acta 1100: 312-320
Nagy L, Bálint E, Barber J, Ringler A, Cook KM and Maróti P (1995) Photoinhibition and law of reciprocity in photosynthetic reactions of Synechocystissp PCC 6803. J Plant Physiol 145: 410-414
Pang Q and Hays JB (1991) UV-B-inducible and temperature-sensitive photoreactivation of cyclobutane pyrimidine dimers in Arabidipsis thaliana. Plant Physiol 95: 536-543
Prasil O, Adir N and Ohad I (1992) Dynamics of Photosystem II: mechanism of photoinhibition and recovery processes. In: Barber J (ed) Topics in Photosynthesis The Photosystems: Structure, Function, and Molecular Biology. Vol 11, pp 295-348. Elsevier, Amsterdam, London, New York, Tokyo
Renger G, Völker M, Eckert HJ, Fromme R, Hohm-Veit S and Graber P (1989) On the mechanism of Photosystem II deterioration by UV-B irradiation. Photochem Photobiol 49: 97-105
Rontó G, Fekete A, Gáspár S and Módos K (1989) Action spectra for photoinduced inactivation of bacteriophage T7 sensitized by 8-methoxypsoralen and angelicin. J Photochem Photobiol 3B: 497-507
Sancar A and Sancar GB (1988) DNA repair enzymes. Annu Rev Biochem 57: 29-67
Setlow RB (1974) The wavelengths in sunlight effective in producing skin cancer: A theoretical analysis. Proc Natl Acad Sci USA 71: 3363-3366
Sinclair J, Park Y-I, Chow WS and Anderson JM (1996) Target theory and the inactivation of Photosystem II. Photosynth Res 50: 33-40
Smith RC, Prézelin BB, Baker KS, Bidigare RR, Boucher NP, Coley T, Karentz D, MacIntyre S, Matlick HA, Menzies D, Ondrusek M, Wan Z and Waters KJ (1995) Ozone depletion: Ultraviolet radiation and phytoplankton biology in antarctic waters. Science 255: 952-959
Spetea C, Hideg É and Vass I (1996) The quinone electron acceptors are not the main senzitizers of UV-B induced protein damage in isolated Photosystem II reaction centre-and core complexes. Plant Sci 115: 207-215
Trebst A and Depka B (1990) Degradation of the D-1 protein subunit of Photosystem II in isolated thylakoids by UV light. Z Naturforsch 45c: 765-771
Vass I (1996) Adverse effects of UV-B light on the structure and function of the photosynthetic apparatus. In: Pessarakli M (ed) Handbook of Photosynthesis, pp 931-950. Marcel Dekker, New York
Vass I, Sass L, Spetea C, Bakou A, Ghanotakis D and Petrouleas V (1996) UV-B induced inhibition of Photosystem II electron transport studied by EPR and chlorophyll fluorescence. Impairment of donor and acceptor side components. Biochemistry 35: 8964-8973
Virgin I, Ghanotakis DF and Andersson B (1990) Light-induced D1-protein degradation in isolated Photosystem II core complexes. FEBS Lett 269: 45-48
Yerkes CT, Kramer DM, Fenton JM and Crofts AR (1990) UV-photoinhibition: Studies in vitro and in intact plants. In: Baltscheffsky M (ed) Current Research in Photosynthesis, Vol II, pp II.6.381-II.6.384. Kluwer Academic Publishers, Dordrecht, the Netherlands
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Sass, L., Spetea, C., Máté, Z. et al. Repair of UV-B induced damage of Photosystem II via de novo synthesis of the D1 and D2 reaction centre subunits in Synechocystis sp. PCC 6803. Photosynthesis Research 54, 55–62 (1997). https://doi.org/10.1023/A:1005895924892
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DOI: https://doi.org/10.1023/A:1005895924892