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Production of optically pure L-alanine in batch and continuous stirred tank reactors using immobilized Pseudomonas sp.

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Abstract

A process to obtain optically pure l-alanine has been developed using batch and continuous stirred tank reactors with a new l-aminoacylase-producing bacterium Pseudomonas sp. BA2 immobilized in calcium alginate beads coated with glutaraldehyde. The maximum production of l-alanine in a continuous stirred tank reactor was 11.26 g after 2 days of operation which is higher than that previously reported.

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Authors and Affiliations

  1. Department of Chemical Engineering, Biochemical Engineering Group, Faculty of Chemistry, University of Murcia, Campus of Espinardo, 30071, Murcia, Spain

    H. Hernández, M.L. Asanza, I. Alcaraz, A.M. Hidalgo, M.F. Máximo & J. Bastida

Authors
  1. H. Hernández
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  2. M.L. Asanza
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  3. I. Alcaraz
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  4. A.M. Hidalgo
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  5. M.F. Máximo
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  6. J. Bastida
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Corresponding author

Correspondence to J. Bastida.

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Hernández, H., Asanza, M., Alcaraz, I. et al. Production of optically pure L-alanine in batch and continuous stirred tank reactors using immobilized Pseudomonas sp.. Biotechnology Letters 23, 887–891 (2001). https://doi.org/10.1023/A:1010524110121

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  • DOI: https://doi.org/10.1023/A:1010524110121

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