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Detection and Characterization of Membrane Microheterogeneity by Resonance Energy Transfer

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Abstract

The application of resonance energy transfer (RET) in the study of heterogeneity in membrane systems is described. Useful formalisms for monophasic and biphasic systems are presented, together with quantitative studies. Evidence for reduction of dimensionality, probe segregation, and microdomain sizes in these systems is discussed. Selected examples of multicomponent systems (natural membranes or model systems including proteins) are also referred, as well as recent work using RET under the microscope.

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Authors and Affiliations

  1. Centro de Química-Física Molecular, Instituto Superior Técnico, P-1049-001, Lisboa, Portugal

    Luís M. S. Loura, Rodrigo F. M. de Almeida & Manuel Prieto

  2. Departamento de Química, Universidade de Évora, Rua Romão Ramalho, 59, P-7000-671, Évora, Portugal

    Luís M. S. Loura

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  1. Luís M. S. Loura
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  2. Rodrigo F. M. de Almeida
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  3. Manuel Prieto
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Loura, L.M.S., de Almeida, R.F.M. & Prieto, M. Detection and Characterization of Membrane Microheterogeneity by Resonance Energy Transfer. Journal of Fluorescence 11, 197–209 (2001). https://doi.org/10.1023/A:1012249117580

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