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The growth and phosphorus utilisation of plants in sterile media when supplied with inositol hexaphosphate, glucose 1-phosphate or inorganic phosphate

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Abstract

Seedlings of six temperate pasture species, three grasses and three legumes, were grown for 19–24 days in sterile agar or sand-vermiculite media, in the presence of inorganic phosphate (Pi), glucose 1-phosphate (G1P) or inositol hexaphosphate (IHP). Agar (pH 5.0) had a low IHP-sorbing capacity while IHP was almost completely sorbed in sand-vermiculite. Pi and G1P were relatively available in both media. Growth of each species was measured in relation to phosphorus (P) supply and levels of Pi supply at which shoot yields reached 90% of maximum yield (Pcrit) were determined. Pcrit values were generally higher for the legume species than for the grasses, and were six-fold higher for Trifolium subterraneum L. seedlings when grown in sand-vermiculite relative to agar. When supplied with G1P, seedlings of the six species grew as well as plants supplied with Pi. By contrast, IHP was a poor source of P for plant growth, even when supplied in agar at levels up to 40-fold greater than Pcrit. Using the growth of T. subterraneum in the presence of IHP, it was calculated that roots released approximately 0.09 nkat phytase g-1 root dry wt per day, over 20 days of growth. By supplementing agar containing IHP with phytase from Aspergillus niger (E.C. 3.1.3.8; 0.012 nkat plant-1, or ∼1.3 nkat g-1 root dry wt), sufficient P became available to enable T. subterraneum seedlings to grow as well as Pi-supplied plants. These results indicate that while pasture plants can quite effectively use P from some organic P sources (e.g. G1P), the acquisition of phytate-P is limited both by availability of substrate and the capacity of plant roots to hydrolyse available IHP.

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References

  • Adams M A and Pate J S 1992 Availability of organic and inorganic forms of phosphorus to lupins (Lupinus spp.). Plant Soil 145, 107–113.

    Article  Google Scholar 

  • Anderson G 1964 Investigations on the analysis of inositol hexaphosphate in soils. In 8th International Congress of Soil Science. Vol. 4. pp. 563–572, Bucharest, Romania.

    Google Scholar 

  • Anderson G 1980 Assessing organic phosphorus in soils. In The Role of Phosphorus in Agriculture. Eds FE Khasawneh, EC Sample and EJ Kamprath. pp. 411–431. American Society of Agronomy, Madison, Wisconsin.

    Google Scholar 

  • Barber S A 1984 Soil Nutrient Bioavailability. A Mechanistic Approach. Wiley-Interscience, New York, USA.

    Google Scholar 

  • Colpaert J V, Van Laere A, Van Tichelen K K and Van Assche J A 1997 The use of inositol hexaphosphate as a phosphorus source by mycorrhizal and non-mychorrhizal Scots Pine (Pinus sylvestris). Func. Ecol. 11, 407–415.

    Article  Google Scholar 

  • Dalal R C 1977 Soil organic phosphorus. Adv. Agron. 29, 83–117.

    CAS  Google Scholar 

  • Findenegg G R and Nelemans J A 1993 The effect of phytase on the availability of P from myo-inositol hexaphosphate (phytate) for maize roots. Plant Soil 154, 189–196.

    Article  CAS  Google Scholar 

  • Hayes J E, Richardson A E and Simpson R J 1999 Phytase and acid phosphatase activities in extracts from roots of temperate pasture grass and legume seedlings. Aust. J. Plant Physiol. 26, 801–809.

    Article  CAS  Google Scholar 

  • Helal H M 1990 Varietal differences in root phosphatase activity as related to the utilization of organic phosphates. In Genetic Aspects of Plant Mineral Nutrition. Eds N El Bassam, M Dambroth and BC Loughman. pp. 103–105. Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • Hübel F and Beck E 1996 Maize root phytase. Purification, characterization and localization of enzyme activity and its putative substrate. Plant Physiol. 112, 1429–1436.

    PubMed  Google Scholar 

  • Jackman R H and Black C A 1951 Solubility of iron, aluminum, calcium and magnesium inositol phosphates at different pH values. Soil Sci. 72, 179–186.

    Article  CAS  Google Scholar 

  • Li M, Osaki M, Honma M and Tadano T 1997a Purification and characterization of phytase induced in tomato roots under phosphorus-deficient conditions. Soil Sci. Plant Nutr. 43, 179–190.

    CAS  Google Scholar 

  • Li M, Osaki M, Rao I M and Tadano T 1997b Secretion of phytase from the roots of several plant species under phosphorusdeficient conditions. Plant Soil 195, 161–169.

    Article  Google Scholar 

  • McKercher R B and Anderson G 1968 Content of inositol penta-and hexaphosphates in some Canadian soils. J. Soil Sci. 19, 47–55.

    CAS  Google Scholar 

  • Murphy J and Riley J P 1962 A modified solution method for the determination of phosphate in natural waters. Anal. Chim. Acta 27, 31–36.

    Article  CAS  Google Scholar 

  • Pérez Corona M E, Van der Klundert I and Verhoeven J T A 1996 Availability of organic and inorganic phosphorus compounds as phosphorus sources for Carex species. New Phytol. 133, 225–231.

    Article  Google Scholar 

  • Pinkerton A, Smith F W and Lewis D C 1997 Pasture Species. In Plant Analysis: An Interpretation Manual, 2nd edn. Eds DJ Reuter and JB Robinson. pp. 287–346. CSIRO Publishing, Collingwood, Australia.

    Google Scholar 

  • Richardson A E and Hadobas P A 1997 Soil isolates of Pseudomonas spp. that utilize inositol phosphates. Can. J. Microbiol. 43, 509–516.

    Article  PubMed  CAS  Google Scholar 

  • Richardson A E, Hadobas P A and Hayes J E 2000 Acid phosphomonoesterase and phytase activities of wheat (Triticum aestivum L.) roots and utilisation of organic phosphorus substrates by seedlings grown in sterile culture. Plant Cell Env. 23 (In press)

  • Ron Vaz M D, Edwards A C, Shand C A and Cresser M S 1993 Phosphorus fractions in soil solution: influence of soil acidity and fertiliser additions. Plant Soil 148, 175–183.

    Article  CAS  Google Scholar 

  • Schoenau J J and Huang W Z 1991 Anion-exchange membrane, water and sodium bicarbonate extractions as soil tests for phosphorus. Commun. Soil Sci. Plant Anal. 22, 465–492.

    Article  CAS  Google Scholar 

  • Seeling B and Jungk A 1996 Utilization of organic phosphorus in calcium chloride extracts of soil by barley plants and hydrolysis by acid and alkaline phosphatases. Plant Soil 178, 179–184.

    Article  CAS  Google Scholar 

  • Tadano T, Ozawa K, Sakai H, Osaki Mand Matsui H 1993 Secretion of acid phosphatase by the roots of crop plants under phosphorusdeficient conditions and some properties of the enzyme secreted by lupin roots. Plant Soil 155/156, 95–98.

    Article  Google Scholar 

  • Tarafdar J C and Marschner H 1994 Efficiency of VAM hyphae in utilisation of organic phosphorus by wheat plants. Soil Sci. Plant Nutr. 40, 593–600.

    CAS  Google Scholar 

  • Tarafdar J C and Marschner H 1995 Dual inoculation with Aspergillus fumigatus and Glomus mossae enhances biomass production and nutrient uptake in wheat (Triticum aestivum L.) supplied with organic phosphorus as Na-phytate. Plant Soil 173, 97–102.

    Article  CAS  Google Scholar 

  • Ullah A H J and Gibson D M 1987 Extracellular phytase (E.C. 3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization. Prep. Biochem. 17, 63–91.

    PubMed  CAS  Google Scholar 

  • Vincent J M 1970 A Manual for the Practical Study of Root-Nodule Bacteria. Blackwell Scientific Publications, Oxford, England.

    Google Scholar 

  • Williams C H and Anderson G 1968 Inositol phosphates in some Australian soils. Aust. J. Soil Res. 6, 121–130.

    Article  CAS  Google Scholar 

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Authors
  1. Julie E. Hayes
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  2. Richard J. Simpson
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  3. Alan E. Richardson
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Hayes, J.E., Simpson, R.J. & Richardson, A.E. The growth and phosphorus utilisation of plants in sterile media when supplied with inositol hexaphosphate, glucose 1-phosphate or inorganic phosphate. Plant and Soil 220, 165–174 (2000). https://doi.org/10.1023/A:1004782324030

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