Abstract
Nitrile hydratase from Rhodococcus sp. DSM 11397 and nitrilase from Pseudomonas. DSM 11387 both retained activity in various organic/aqueous biphasic mixtures. Both enzymes were most tolerant (66–109% retained activity) of C8 and C16 alkanes with log P values greater than 4.0. Some enzyme activity (<10% nitrile hydratase, <60% nitrilase) was also retained in monophasic water-saturated C6-C11 n-alkanols. © Rapid Science Ltd. 1998
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Layh, N., Willetts, A. Enzymatic nitrile hydrolysis in low water systems. Biotechnology Letters 20, 329–331 (1998). https://doi.org/10.1023/A:1005358809561
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DOI: https://doi.org/10.1023/A:1005358809561