Abstract
Fractional precipitation is a simple and efficient procedure for pre-purification of paclitaxel. The optimal methanol composition in water, paclitaxel content in crude extract, storage temperature, and time were 61.5% (v/v), 0.5% (w/v), 0 °C, and 3 days, respectively. As purity of the paclitaxel in the crude extract increases, the purity and yield of paclitaxel from fractional precipitation increases. This pre-purification process serves to minimize the solvent usage, size and complexity of the HPLC operations for paclitaxel purification. This process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced.
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References
Cardellina II, JH (1991) HPLC separation of taxol and cephalomannine. J. Liq. Chromatogr. 14: 659–665.
Carver DR, Prout TR, Workman CT, Hughes CL (1996) Reverse osmosis and ultrafiltration methods for solutions to isolate desired solutes including taxane. U.S. Patent 5,549,830.
Castor TP (1998) Method and apparatus for isolating therapeutic compositions from source materials. U.S. Patent 5,750,709.
Castor TP, Tyler TA (1993) Determination of taxol in Taxus media needles in the presence of interfering compounds. J. Liq.Chromatogr. 16: 723–731.
Choi HK, Adams TL, Stahlhut RW, Kim SI, Yun JH, Song BK, Kim JH, Hong SS, Lee HS (1999) Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture. U.S. Patent 5,871,979.
ElSohly HN, Croom EM, ElSohly MA, McChesney JD (1997) Methods and compositions for isolating taxanes. U.S. Patent 5,618,538.
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158.
Hong SS, Song BK, Kim JH, Lim CB, Lee HS, Kim KW, Kang IS, Park HB (1999) Method for purifying taxol from Taxus biomass. U.S. Patent 5,900,979.
Kim DJ, Chang HN (1990) Enhanced shikonin production from Lithospermum erythrorhizon by in situ extraction and calcium alginate immobilization. Biotechnol. Bioeng. 36: 460–466.
Ma W, Park GL, Gomez GA, Nieder MH, Adams TL, Aynsley JS, Sahai OP, Smith RJ, Stahlhut RW, Hylands PJ (1994) New bioactive taxoids from cell cultures of Taxus baccata. J. Nat. Prod. 57: 116–122.
Nair MG (1995) Process for the isolation and purification of taxol and taxanes from Taxus using methanol or acetone. U.S. Patent 5,478,736.
Rao KV (1997) Method for the isolation and purification of taxol and its natural analogues. U.S. Patent 5,670,673.
Witherup KM, Look SA, Stasko MW, Ghiorzi TJ, Muschik GM (1990) Taxus spp. needles contain amounts of taxol comparable to the bark of Taxus brevifolia: analysis and isolation. J. Nat. Pro 53: 1249–1255.
Wu DJ, Ma Z, Wang NHL (1999) Optimization of throughput and desorbent in SMB chromatography for paclitaxel purification. J. Chromatogr. A. 855: 71–89.
Zhang Z, Jia Z (1991) Taxanes from Taxus chinensis. Phytochem. 30: 2345–2348.
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Kim, JH., Kang, IS., Choi, HK. et al. Fractional precipitation for paclitaxel pre-purification from plant cell cultures of Taxus chinensis . Biotechnology Letters 22, 1753–1756 (2000). https://doi.org/10.1023/A:1005642001815
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DOI: https://doi.org/10.1023/A:1005642001815