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Proofs of the Structure of Lipid Coated Nanoparticles (SMBV™) Used as Drug Carriers

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Abstract

Purpose. Supramolecular Biovectors (SMBV™) consist of cross-linkedcationic nanoparticles surrounded by a lipid membrane. Thepurpose was to study the structure of the lipid membrane and tocharacterise its interaction with the nanoparticles in order to differentiateSMBV™ from other polymer/lipid associations.

Methods. The interaction of lipids with the nanoparticle surface wasstudied using zeta potential, Fluorescence Energy Transfer (FET) andFluorescence Microscopy. SMBV™ were compared to liposomes andmixtures nanoparticles/liposomes. Finally the structure of SMBV™was visualised by Electron Microscopy.

Results. Zeta potential measurements showed that lipids on SMBV™had a pronounced shielding effect on the surface charge. This was notthe case for mixtures of nanoparticles and liposomes. FET experimentsconfirmed these results indicating that, for SMBV™, the lipids aremuch closer to the nanoparticle surface. SMBV™ Fluorescence microscopyon model microparticles showed a lipid crown on SMBV™ thatwas confirmed by electron microscopy on SMBV™ nanoparticles.

Conclusions. Results show that in case of SMBV™ lipids are stronglyadsorbed on the polysaccharide core surface probably due to ionic/hydrophobicinteractions. The resulting supramolecular structure is aspherical cationic polysaccharide particle surrounded by a phospholipid/cholesterol layer.

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Authors and Affiliations

  1. Biovector Therapeutics S.A., Chemin du Chene Vert, 31676, Labege Cedex, France

    Ignacio De Miguel

  2. Biovector Therapeutics S.A., Chemin du Chene Vert, 31676, Labege Cedex, France

    Laurent Imbertie, Valerie Rieumajou, Michel Major, Roger Kravtzoff & Didier Betbeder

Authors
  1. Ignacio De Miguel
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  2. Laurent Imbertie
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  3. Valerie Rieumajou
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  4. Michel Major
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  5. Roger Kravtzoff
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  6. Didier Betbeder
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De Miguel, I., Imbertie, L., Rieumajou, V. et al. Proofs of the Structure of Lipid Coated Nanoparticles (SMBV™) Used as Drug Carriers. Pharm Res 17, 817–824 (2000). https://doi.org/10.1023/A:1007504124603

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